Microgeographic population genetic structure in the northern water snake, Nerodia sipedon sipedon detected using microsatellite DNA loci
Microgeographic population genetic structure in the northern water snake, Nerodia sipedon sipedon detected using microsatellite DNA loci
复制标题
利用微卫星DNA位点检测北方水蛇Nerodia sipedon sipedon的微地理种群遗传结构
作者：
M. Prosser;H. Lisle Gibbs;P. J. Weatherhead
我们描述了北方水蛇 Nerodia sipedon sipedon 的 8 个微卫星 DNA 基因座的分离和遗传特征，并使用这些基因座来分析安大略省这些蛇的当地（相距 <2 公里）种群之间的遗传分化水平。这些位点是可变的，预期杂合度范围为 0.28 至 0.91，并且可以在亲子分析中以很高的概率 (0.998) 正确排除非父亲。群体分析揭示了一个群体与预期杂合性水平的显着偏差，这可能是单个基因座处的无效等位基因以及所有三个群体之间小但显着水平的遗传分化的结果。这表明该物种存在微观地理遗传结构，可能是由于有限的扩散。
We describe the isolation and genetic characterization of eight microsatellite DNA loci from the northern water snake, Nerodia sipedon sipedon and use these loci to analyse levels of genetic differentiation between local (<2km apart) populations of these snakes in Ontario. These loci are variable, with expected heterozygosities ranging from 0.28 to 0.91, and can correctly exclude nonsires in parentage analyses with a high probability (0.998). Population analyses reveal significant deviation from expected heterozygosity levels for one population, probably a result of a null allele(s) at a single locus and small but significant levels of genetic differentiation among all three populations. This demonstrates that microgeographic genetic structure exists in this species, possibly due to limited dispersal.