Suppressive effects of Anoectochilus formosanus extract on osteoclast formation in vitro and bone resorption in vivo
Suppressive effects of Anoectochilus formosanus extract on osteoclast formation in vitro and bone resorption in vivo
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DOI：
10.1007/s00774-007-0810-8
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发表时间：
2008-03-01
影响因子：
3.3
通讯作者：
Yazawa, Kazunaga
中科院分区：
文献类型：
--
作者：
Masuda, Kikuko;Ikeuchi, Mayumi;Yazawa, Kazunaga
台湾金线莲是一种原产于台湾的植物，被用作民间药物。结果表明，口服A.本研究以台湾产植物萃取物（AFE）（500 mg/kg）治疗骨质疏松症实验模型--卵巢切除小鼠，持续4周，可抑制其骨重量及骨小梁的流失。尽管12.5和25 μ g/ml的AFE抑制成骨细胞和骨髓细胞共培养中破骨细胞的形成，但AFE不抑制骨髓细胞和RAW 264细胞中破骨细胞祖细胞和前破骨细胞的形成。然而，AFE（12.5和25 μ g/ml）降低RANKL表达。这些结果表明，AFE可能通过抑制破骨细胞形成所需的RANKL表达来抑制雌激素缺乏引起的骨丢失。
Anoectochilus formosanus, a plant native to Taiwan, is used as a folk medicine. It was found that oral administration of A. formosanus extract (AFE) (500 mg/kg) for 4 weeks suppressed bone weight loss and trabecular bone loss in ovariectomized mice, an experimental model of osteoporosis. Although AFE at 12.5 and 25 mu g/ml inhibited osteoclast formation in co-culture of osteoblasts and bone marrow cells, AFE did not inhibit the formation of osteoclast progenitor cells and preosteoclast cells in bone marrow cells and RAW264 cells. However, AFE (at 12.5 and 25 mu g/ml) decreased RANKL expression. These results suggested that AFE might suppress the bone loss caused by estrogen deficiency through suppression of RANKL expression required for osteoclast formation.