A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue.
A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue.
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DOI：
10.1155/2014/768136
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发表时间：
2014
影响因子：
3.1
通讯作者：
Blom K
中科院分区：
文献类型：
--
作者：
Hakonen B;Lönnberg LK;Larkö E;Blom K
缺乏可预测的体外方法来分析抗菌活性可能在抗生素耐药性的发展中发挥作用。目前使用的方法分析嗜酸性细胞，但为了使该方法具有临床相关性，应该研究体内类似条件下的生物膜。因此，我们的小组已经开发了一种定性和定量的方法，在体内类似的3D组织预测的抗菌活性在现实中。将器械（伤口敷料）应用于铜绿假单胞菌接种的Muller-Hinton（MH）琼脂或3D合成软组织（SST）上，并孵育24小时。然后通过目视和活菌计数分析抗菌活性。在MH琼脂上，三个含银器械中有两个显示抑制区（ZOI），在SST上，所有三个器械均检测到ZOI。在评价SST中的细菌负荷时发现了确证结果，并显示其具有银浓度依赖性。总之，开发了一种新的方法，结合组织和器械中抗菌活性的目视快速筛选和定量评价。它使用允许生物膜形成的组织，从而密切模仿现实。这些条件对于在预防器械相关感染的任务中预测医疗器械的抗菌活性至关重要。
The lack of predictable in vitro methods to analyze antimicrobial activity could play a role in the development of resistance to antibiotics. Current used methods analyze planktonic cells but for the method to be clinically relevant, biofilm in in vivo like conditions ought to be studied. Hence, our group has developed a qualitative and quantitative method with in vivo like 3D tissue for prediction of antimicrobial activity in reality. Devices (wound dressings) were applied on top of Pseudomonas aeruginosa inoculated Muller-Hinton (MH) agar or 3D synthetic soft tissues (SST) and incubated for 24 hours. The antibacterial activity was then analyzed visually and by viable counts. On MH agar two out of three silver containing devices showed zone of inhibitions (ZOI) and on SST, ZOI were detected for all three. Corroborating results were found upon evaluating the bacterial load in SST and shown to be silver concentration dependent. In conclusion, a novel method was developed combining visual rapid screening and quantitative evaluation of the antimicrobial activity in both tissue and devices. It uses tissue allowing biofilm formation thus mimicking reality closely. These conditions are essential in order to predict antimicrobial activity of medical devices in the task to prevent device related infections.