Active and total prokaryotic communities in dryland soils
Active and total prokaryotic communities in dryland soils
复制标题
DOI：
10.1111/1574-6941.12155
复制
发表时间：
2013-10-01
影响因子：
4.2
通讯作者：
Gillor, Osnat
中科院分区：
文献类型：
--
作者：
Angel, Roey;Pasternak, Zohar;Gillor, Osnat
土壤微生物群落总数与代谢活性之间的关系随环境变化而发生显著变化。在干旱地区，水的可用性是限制细胞活动的关键因素。我们调查了从干旱沙漠到地中海森林的土壤中总的和活跃的微生物和细菌的多样性。三十复合土壤样品被检索从五个站点沿着的降水梯度，收集补丁位于之间和下的优势多年生植物在每个站点。利用分子指纹技术，根据16 S rRNA基因（总群落）和PCR或RT-PCR扩增的rRNA基因（活性群落）对群落进行定位排序。土壤样品之间的差异要高得多，而不是活跃的微生物群落：不同地点之间的DNA指纹的差异是1.2和2.5倍高于RNA差异（分别为细菌和细菌）。DNA指纹之间的斑块类型差异平均比RNA差异大2.7-19.7倍。此外，基于RNA的群落格局与土壤水分高度相关，但不一定遵循空间分布格局。我们的研究结果表明，在水资源有限的环境中，通过分析活跃的社区所获得的空间格局不像从总社区得出的那样强大。
The relationship between total and metabolically active soil microbial communities can change drastically with environment. In dry lands, water availability is a key factor limiting cells' activity. We surveyed the diversity of total and active Archaea and Bacteria in soils ranging from arid desert to Mediterranean forests. Thirty composited soil samples were retrieved from five sites along a precipitation gradient, collected from patches located between and under the dominant perennial plant at each site. Molecular fingerprinting was used to site-sort the communities according of their 16S rRNA genes (total community) and their rRNA (active community) amplified by PCR or RT-PCR from directly extracted soil nucleic acids. The differences between soil samples were much higher in total rather than active microbial communities: differences in DNA fingerprints between sites were 1.2 and 2.5 times higher than RNA differences (for Archaea and Bacteria, respectively). Patch-type discrepancies between DNA fingerprints were on average 2.7-19.7 times greater than RNA differences. Moreover, RNA-based community patterns were highly correlated with soil moisture but did not necessarily follow spatial distribution pattern. Our results suggest that in water-limited environments, the spatial patterns obtained by the analysis of active communities are not as robust as those drawn from total communities.