Single-cell patterning: a new frontier in bioengineering
Single-cell patterning: a new frontier in bioengineering
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DOI：
10.1016/j.mtchem.2022.101021
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发表时间：
2022-07-03
影响因子：
7.3
通讯作者：
Santra, T. S.
中科院分区：
文献类型：
--
作者：
Gayathri, R.;Kar, S.;Santra, T. S.
单细胞图案化技术正在迅速发展，其中单细胞定位在空间上不同的区域中，从而为多种生物学和生物医学、治疗学和诊断学应用提供平台。通过考虑细胞异质性并提供更多的统计能力，单细胞图案化比传统的整体图案化更好地进行精确的细胞分析，传统的整体图案化可以用数百万个细胞一起获得平均数据。细胞图案化中的关键步骤是通过适应用于基底修饰和图案化的几种物理和生物化学策略来调节基底上的细胞粘附。在这篇文章中，我们简要地讨论了不同的生物化学以及物理方法的表面改性和细胞粘附。此外，我们还强调通过使用不同的物理化学和物理方法及其生物学和生物医学应用进行深入的单细胞图案化。最后，单细胞打印，它的优点，局限性和未来的挑战也进行了阐述。
Single-cell patterning technologies are rapidly evolving, where single cells are positioned in spatially distinct areas, thus providing a platform for several biological and biomedical, therapeutics, and diagnostics applications. By considering the cellular heterogeneity and providing more statistical power, single-cell patterning outperforms precise cellular analysis than conventional bulk patterning, where average data can be acquired with millions of cells together. A crucial step in cell patterning is to regulate cell adhesion on a substrate by adapting several physical and biochemical strategies for substrate modification and patterning. In this article, we briefly discuss different biochemical as well as physical approaches for the surface modification and cellular adhesion. Moreover, we also emphasize in-depth single-cell patterning by using different physiochemical and physical approaches and their biological and biomedical applications. Finally, single-cell printing, its advantages, limitations, and future challenges are also elaborated.