Upconversion Nanocrystals Mediated Lateral-Flow Nanoplatform for in Vitro Detection
Upconversion Nanocrystals Mediated Lateral-Flow Nanoplatform for in Vitro Detection
复制标题
用于体外检测的上转换纳米晶体介导的横向流动纳米平台
DOI：
10.1021/acsami.6b14906
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发表时间：
2017-02-01
影响因子：
9.5
通讯作者：
Zhou, Lei
中科院分区：
文献类型：
--
作者：
Liang, Zhiqin;Wang, Xiaochen;Zhou, Lei
上转换发光材料不受生物样品自身荧光的干扰，在体内和体外生物应用方面受到了广泛的关注。然而，要实现广泛的应用，UCPs需要是水分散的、纳米级的和高度发光的。因此，本研究的目的是开发符合这些综合标准的体外诊断UCPs。为了将纳米尺寸和高发光强度结合起来，制备了Li+和K+共掺杂的β-NaYF4：Yb3+，Er3+上转换纳米晶(UCNPs)，它具有较高的上转换强度和较小的尺寸。与未掺杂的NaYF4：Yb3+，Er3+纳米晶相比，Na0.9Li0.07K0.03YF4：Yb3+，Er3+纳米晶的最强绿光发射和红光发射分别提高了7倍和10倍。一种温和的溶胶-凝胶表面修饰方法被用来制备水相分散体并允许共价生物分子连接。将活化的UCNPs作为生物报告分子，与经典的侧向流动试验相结合，建立了一种同时检测鼠疫耶尔森菌和假鼻疽伯克霍尔德氏菌的双靶点检测方法。该方法的灵敏度为10(3)cfu/test，两个靶点之间没有交叉干扰。本研究提供了一种制备具有综合性能的UCNPs的方法，用于在体内和体外生物应用中作为优异的光学报告程序。
Upconversion phosphors (UCPs) that are free from interference from biological sample autofluorescence have attracted attention for in vivo and in vitro bioapplications. However, UCPs need to be water-dispersible, nanosized, and highly luminous to realize broad applications. Therefore, the aim of this research is to develop UCPs that meet these comprehensive criteria for in vitro diagnosis. To combine nano size with high luminous intensity, beta-NaYF4:Yb3+,Er3+ upconversion nanocrystals (UCNPs) codoped with Li+ and K+ are prepared that display high upconversion intensities as well as small size. The strongest green and red emissions of the Na0.9Li0.07K0.03YF4:Yb3+,Er3+ nanocrystals are increased by 7 and 10 times, respectively, compared with those of the undoped NaYF4:Yb3+,Er3+ nanocrystals. A mild sol-gel surface modification method is used to produce water -phase dispersions and allow covalent biomolecule conjugation. The bioactivated UCNPs are used as a bioreporter and integrated with a classical lateral flow assay to establish an assay to accomplish simultaneous dual-target detection of Yersinia pestis and Burkholderia pseudomallei. The assay achieves a sensitivity of 10(3) CFU/test without cross-interference between two targets. The research provides a way to produce UCNPs with comprehensive properties for use as excellent optical reporters in in vivo and in vitro bioapplications.