FMRFAMIDE-RELATED PEPTIDES IN THE SEX SEGMENTAL GANGLIA OF THE PHARYNGOBDELLID LEECH ERPOBDELLA-OCTOCULATA - IDENTIFICATION AND INVOLVEMENT IN THE CONTROL OF HYDRIC BALANCE
FMRFAMIDE-RELATED PEPTIDES IN THE SEX SEGMENTAL GANGLIA OF THE PHARYNGOBDELLID LEECH ERPOBDELLA-OCTOCULATA - IDENTIFICATION AND INVOLVEMENT IN THE CONTROL OF HYDRIC BALANCE
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DOI：
10.1111/j.1432-1033.1994.tb18738.x
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发表时间：
1994-04-01
期刊：
影响因子：
--
通讯作者：
MALECHA, J
中科院分区：
文献类型：
--
作者：
SALZET, M;BULET, P;MALECHA, J
Using enzyme-linked immunosorbent assays, a dot-immunobinding assay and a three-step reverse-phase HPLC separation, four Arg-Phe-amide (RFamide) peptides were purified from sex segmental ganglia extracts of the leech Erpobdella octoculata; FMRFamide, FM(O)RFamide, FLRFamide and GDPFLRFamide. Their amino acid sequences were elucidated by means of a combined approach using antiserum specificity, synthetic-peptide coelution, automated Edman degradation and electrospray mass spectrometry. One of these peptides, GDPFLRFamide, is a novel leech RFamide neuropeptide.Two of the above RFamide peptides are involved in the control of leech hydric balance; one (GDPFLRFamide) is diuretic, the other (FMRFamide) is anti-diuretic.Titration of each purified RFamide peptide indicated a similar amount of each tetrapeptide and of tetrapeptides and heptapeptides. A comparison between RFamide peptides of E. octoculata and molluscs reveals structural similarities supporting the hypothesis for the existence of an ancestral RFamide peptide gene common to leeches and molluscs.
