Association of Circulating Level of High Mobility Group Box 1 With Disease Activity in Antineutrophil Cytoplasmic Autoantibody-Associated Vasculitis
Association of Circulating Level of High Mobility Group Box 1 With Disease Activity in Antineutrophil Cytoplasmic Autoantibody-Associated Vasculitis
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DOI：
10.1002/acr.22187
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发表时间：
2013-11-01
影响因子：
4.7
通讯作者：
Chen, Min
中科院分区：
文献类型：
--
作者：
Wang, Chen;Gou, Shen-Ju;Chen, Min
目的高迁移率组框1 （HMGB-1）是一种促炎介质，与炎症和组织损伤有关。既往研究报道，活动性抗中性粒细胞胞浆自身抗体（ANCA）相关血管炎（AAV）患者的循环HMGB-1水平与肾脏表现和肉芽肿性炎症负担相关。本研究旨在探讨AAV中循环HMGB-1水平是否与疾病活动性相关。方法收集74例AAV活动性患者和65例AAV缓解期患者的血浆样本。采用酶联免疫吸附法测定血浆HMGB-1水平。分析血浆HMGB-1水平与临床和病理参数的关系。结果活动性AAV患者血浆HMGB-1水平显著高于正常对照组和缓解期AAV患者(中位数为6.11[四分位间距（IQR） 3.25 ~ 12.79] ng/ml vs中位数为1.12 [IQR 0.53 ~ 1.39] ng/ml， P< 0.001；中位数为6.11 [IQR 3.25-12.79] ng/ml，中位数为3.04 [IQR 1.97-4.63] ng/ml， P均< 0.001)。相关分析显示，血浆HMGB-1水平与初始血清肌酐（r = 0.275, P = 0.018）、肾小球滤过率（r = -0.277, P = 0.017）、伯明翰血管炎活动评分（r = 0.308, P = 0.008）和c反应蛋白水平（r = 0.309, P = 0.008）相关。髓过氧化物酶(MPO)-ANCA患者中，血浆HMGB-1水平在第1四分位数的患者MPO-ANCA水平明显低于其他3四分位数的患者。结论血液HMGB-1水平可反映AAV血管炎的疾病活动性和肾脏受累情况。
ObjectiveHigh mobility group box 1 (HMGB-1), a kind of proinflammatory mediator, is associated with inflammatory conditions and tissue damage. Previous studies have reported that circulating HMGB-1 levels in patients with active antineutrophil cytoplasmic autoantibody (ANCA)-associated vasculitis (AAV) were associated with renal manifestations and burdens of granulomatous inflammation. The current study aimed to investigate whether circulating HMGB-1 levels were associated with disease activity in AAV.MethodsPlasma samples from 74 patients with AAV in active stage and 65 patients with AAV in remission were collected. The plasma levels of HMGB-1 were determined by enzyme-linked immunosorbent assay. Associations between plasma levels of HMGB-1 with clinical and pathologic parameters were analyzed.ResultsPlasma levels of HMGB-1 in active AAV patients were significantly higher than those in normal controls and AAV patients in remission (median 6.11 [interquartile range (IQR) 3.25-12.79] ng/ml versus median 1.12 [IQR 0.53-1.39] ng/ml, P< 0.001; median 6.11 [IQR 3.25-12.79] ng/ml versus median 3.04 [IQR 1.97-4.63] ng/ml, P < 0.001, respectively). Correlation analysis showed that plasma levels of HMGB-1 correlated with initial serum creatinine (r = 0.275, P = 0.018), estimated glomerular filtration rate (r = -0.277, P = 0.017), the Birmingham Vasculitis Activity Score (r = 0.308, P = 0.008), and C-reactive protein level (r = 0.309, P = 0.008). Among the patients with myeloperoxidase (MPO)-ANCA, those within the first quartile of plasma HMGB-1 levels had a significantly lower level of MPO-ANCA than those within the other 3 quartiles.ConclusionCirculating HMGB-1 levels might reflect the disease activity and renal involvement of AAV vasculitis.