Native phasing of x-ray free-electron laser data for a G protein-coupled receptor.
Native phasing of x-ray free-electron laser data for a G protein-coupled receptor.
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DOI：
10.1126/sciadv.1600292
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发表时间：
2016-09
期刊：
影响因子：
13.6
通讯作者：
Cherezov V
中科院分区：
文献类型：
--
作者：
Batyuk A;Galli L;Ishchenko A;Han GW;Gati C;Popov PA;Lee MY;Stauch B;White TA;Barty A;Aquila A;Hunter MS;Liang M;Boutet S;Pu M;Liu ZJ;Nelson G;James D;Li C;Zhao Y;Spence JC;Liu W;Fromme P;Katritch V;Weierstall U;Stevens RC;Cherezov V
大多数蛋白质中存在的硫原子的异常信号被用于XFEL数据的从头计算和GPCR结构的求解。连续飞秒晶体学(SFX)利用X射线自由电子激光(XFELs)产生的极亮和超短脉冲，允许在室温下以最小的辐射损害从微米级晶体中收集高分辨率的衍射强度，使用“先衍射后销毁”的原则。然而，到目前为止，用XFELS测定从头结构因子相一直是困难的。我们展示了使用来自自然硫原子的异常信号来解决用XFEL收集的SFX数据的晶体相问题的能力，从而导致人类A2A腺苷受体在1.9ä分辨率下的无偏置室温结构。这一进展是由于SFX数据分析的最新改进，以及用于流动水合微晶的注射器和输送介质的设计。这种通用的方法将加速新型难结晶大分子及其络合物的结构研究。
Anomalous signal from sulfur atoms present in most proteins was used for de novo phasing of XFEL data and solving a GPCR structure. Serial femtosecond crystallography (SFX) takes advantage of extremely bright and ultrashort pulses produced by x-ray free-electron lasers (XFELs), allowing for the collection of high-resolution diffraction intensities from micrometer-sized crystals at room temperature with minimal radiation damage, using the principle of “diffraction-before-destruction.” However, de novo structure factor phase determination using XFELs has been difficult so far. We demonstrate the ability to solve the crystallographic phase problem for SFX data collected with an XFEL using the anomalous signal from native sulfur atoms, leading to a bias-free room temperature structure of the human A2A adenosine receptor at 1.9 Å resolution. The advancement was made possible by recent improvements in SFX data analysis and the design of injectors and delivery media for streaming hydrated microcrystals. This general method should accelerate structural studies of novel difficult-to-crystallize macromolecules and their complexes.
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DOI：
10.1107/s139900471501857x
发表时间：
2015-12-01
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Nakane T;Song C;Suzuki M;Nango E;Kobayashi J;Masuda T;Inoue S;Mizohata E;Nakatsu T;Tanaka T;Tanaka R;Shimamura T;Tono K;Joti Y;Kameshima T;Hatsui T;Yabashi M;Nureki O;Iwata S;Sugahara M
通讯作者：
Sugahara M
DOI：
10.1126/science.1244142
发表时间：
2013-12-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Liu W;Wacker D;Gati C;Han GW;James D;Wang D;Nelson G;Weierstall U;Katritch V;Barty A;Zatsepin NA;Li D;Messerschmidt M;Boutet S;Williams GJ;Koglin JE;Seibert MM;Wang C;Shah ST;Basu S;Fromme R;Kupitz C;Rendek KN;Grotjohann I;Fromme P;Kirian RA;Beyerlein KR;White TA;Chapman HN;Caffrey M;Spence JC;Stevens RC;Cherezov V
通讯作者：
Cherezov V
影响因子：
2.5
作者：
Blaj, Gabriel;Caragiulo, Pietro;Weaver, Matt
通讯作者：
Weaver, Matt
影响因子：
56.9
作者：
Liu, Qun;Dahmane, Tassadite;Hendrickson, Wayne A.
通讯作者：
Hendrickson, Wayne A.
影响因子：
64.8
作者：
Barends, Thomas R. M.;Foucar, Lutz;Schlichting, Ilme
通讯作者：
Schlichting, Ilme