Characterization of 1-Alkenyl-sn-glycero-3-phosphorylcholine Acyltransferase Activity in the Microsomes from Rat Parotid Gland
Characterization of 1-Alkenyl-sn-glycero-3-phosphorylcholine Acyltransferase Activity in the Microsomes from Rat Parotid Gland
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大鼠腮腺微粒体中 1-烯基-sn-甘油-3-磷酸胆碱酰基转移酶活性的表征
DOI：
10.2330/joralbiosci1965.42.319
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
A. Shinkai
中科院分区：
文献类型：
--
作者：
Y. Kameyama;A. Shinkai
醚连接磷脂的烷基和1-烯基（缩醛磷脂）类型几乎仅作为磷脂酰胆碱和磷脂酰乙醇胺的结构类似物存在于哺乳动物细胞中，这是主要的膜磷脂成分1）。烷基型主要以胆碱磷酸甘油脂的形式存在，而缩醛磷脂主要以乙醇胺磷酸甘油脂的形式存在1）。最近，烷基和烯基型磷脂的许多特殊生理功能已被阐明2）。烷基型磷脂类血小板活化因子的研究不仅有重大发现和进展，（PAF：1-烷基-2乙酰基-sn-甘油基-3-磷酰胆碱）3）和抗肿瘤醚脂质（AELs）4，5），而且烯基型磷脂也显示在某些蛋白激酶C同工酶的调节中起作用6，7），可能在膜-膜融合事件中发挥作用8，9）和作为内源性抗氧化剂的能力10，11）。也有人提出，烯基甘油磷脂可作为多不饱和脂肪酸的储存库，12，13）当被钙非依赖性、血浆酶原特异性的磷脂酶A2释放时，可形成生物活性分子，如白藜芦醇和白三烯14）。在下颌下和舌下唾液腺中，醚连接磷脂的分析及其合成酶的特征已被阐明15）。然而，关于腮腺中醚连接磷脂的信息很少。最近，报道了膜磷脂组成及其物理性质的分析，并且显示在大鼠腮腺中存在三种类型的磷脂酰胆碱，1-酰基-2-酰基、1-烯基-2-酰基和1-烷基-2-酰基16 -18）。在本研究中，磷脂重塑酶，酰基转移酶，其特征在于，并提出了可能的机制，丰富的多不饱和脂肪酸胆碱缩醛磷脂。
The alkyl and alk-1-enyl (plasmalogen) types of ether-linked phospholipids are present in mammalian cells almost exclusively as structural analogues of phosphatidylcholine and phosphatidylethanolamine, which are major membrane phospholipid compositions1). The alkyl type is found predominantly as choline phosphoglycerolipids, whereas plasmalogen occurs mainly as ethanolamine phosphoglycerolipids1). Recently, many specific physiological functions of alkyl and alkenyl-type phospholipids have been reported2). Not only have significant discoveries and advancements in the studies of alkyl type phospholipid like platelet activating factor (PAF: 1-alkyl-2acetyl-sn-glycero-3-phosphorylcholine)3) and antitumor ether lipids (AELs)4,5) been reported, but also the alkenyl type of phospholipids have been shown to play a role in the regulation of certain protein kinase C isozymes6,7), a possible role in membrane-membrane fusion events8,9) and the ability to serve as endogenous antioxidants10,11). It has also been suggested that alkenyl glycerophospholipids serve as a reservoir for polyunsaturated fatty acids,12,13) which, when released by a calcium-independent, plasmalogenspecific, phospholipase A2, can form bioactive molecules such as prostaglandins and leukotrienes14). In the submandibular and sublingual salivary glands, the analyses of ether-linked phospholipids and the characterizations of their synthesizing enzymes have been reported15). However, there is very little information about ether-linked phospholipids in the parotid glands. Recently, analyses of membrane phospholipid compositions and their physical properties were reported, and it was shown that there are three types of phosphatidylcholines, 1-acyl-2-acyl, 1-alkenyl-2-acyl and 1-alkyl-2-acyl, in the rat parotid gland16-18). In the present study, a phospholipid-remodelling enzyme, acyltransferase, was characterized and the possible mechanism of enriching polyunsaturated fatty acid in choline plasmalogen was suggested.
DOI：
10.1016/s0021-9258(18)38000-1
发表时间：
1988-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Photosensitized Killing;Raphael;-A.;ZoellerS;Olivier;-H.;MorandQ;Christian R. H Raetzll
通讯作者：
Photosensitized Killing;Raphael;-A.;ZoellerS;Olivier;-H.;MorandQ;Christian R. H Raetzll
缩醛磷脂状态影响巨噬细胞系中的二十二碳六烯酸水平。
影响因子：
6.5
作者：
Gaposchkin,DP;Zoeller,RA
通讯作者：
Zoeller,RA
兔心肌缺血期间膜相关磷脂酶 A2 的激活，对缩醛磷脂底物具有高度选择性。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hazen,SL;Ford,DA;Gross,RW
通讯作者：
Gross,RW