CELL-TYPE SPECIFICITY OF IMMUNOGLOBULIN GENE-EXPRESSION IS REGULATED BY AT LEAST 3 DNA-SEQUENCE ELEMENTS
CELL-TYPE SPECIFICITY OF IMMUNOGLOBULIN GENE-EXPRESSION IS REGULATED BY AT LEAST 3 DNA-SEQUENCE ELEMENTS
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DOI：
10.1016/s0092-8674(85)80069-6
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
--
作者：
GROSSCHEDL, R;BALTIMORE, D
IG μ的细胞类型特异性的调节通过将含有IG基因序列与组织非特异性转录单位的部分结合的各种杂交基因导入淋巴和非淋巴细胞来检查H链（H）基因表达。用病毒增强子替换淋巴细胞特异性IgH增强子不影响μ的组织特异性。IG基因表达。发现了两个新的调节区域，可提供转录组织特异性。首先，显示位置-154和+57之间的VH启动子区指导细菌gpt基因的淋巴细胞特异性转录，甚至在病毒增强子存在下。第二，μ。发现缺乏IgH增强子的基因内序列以组织特异性方式调节积累的IG转录物的水平。IG基因表达的组织特异性不仅受增强子的调控，而且受启动子和尚未确定的基因内序列的调控，其中包含淋巴特异性调控信息。
The regulation of cell-type specificity of Ig .mu. H chain (H) gene expression was examined by introducing various hybrid genes containing Ig gene sequences combined with portions of a tissue-nonspecific transcription unit into lymphoid and nonlymphoid cells. Replacing the lymphocyte-specific IgH enhancer with a viral enhancer did not affect tissue specificity of .mu. Ig gene expression. Two new regulatory regions were identified that provide transcriptional tissue specificity. First, the VH promoter region between position -154 and +57 was shown to direct lymphocyte-specific transcription of a bacterial gpt gene, even in the presence of a viral enhancer. Second, .mu. intragenic sequences, lacking the IgH enhancer, were found to regulate the level of accumulated Ig transcripts in a tissue-specific fashion. Tissue specificity of Ig gene expression is not solely regulated by the enhancer but that the promoter, and as yet undefined intragenic sequences, contain lymphoid-specific regulatory information.