Beyond plant lectin histochemistry: Preparation and application of markers to visualize the cellular capacity for protein-carbohydrate recognition
Beyond plant lectin histochemistry: Preparation and application of markers to visualize the cellular capacity for protein-carbohydrate recognition
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DOI：
10.3109/10520299809141120
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发表时间：
1998-09-01
影响因子：
1.6
通讯作者：
Kayser, K
中科院分区：
文献类型：
--
作者：
Gabius, HJ;Unverzagt, C;Kayser, K
低聚糖可以储存生物信息。在这方面，它们的能力甚至超过核苷酸和氨基酸的寡聚和聚合结构，因此蛋白质-碳水化合物的相互作用被认为参与调节各种细胞活动。几十年来，植物凝集素已被证明在评估各种碳水化合物表位的结构方面以及监测空间和/或时间限制的表达模式方面具有价值。如果这些表位的存在及其发生的改变具有生理相关性，一种合理的可能性是，可视化的糖类在有效的蛋白质-碳水化合物识别系统中充当配体。为了支持这一假设的有效性，糖化合物的受体位点必须被定位。载体固定化碳水化合物（新糖缀合物）足以满足这一目的。化学合成可以利用这种探针。在第一阶段，确定组织中凝集素等结合位点的存在。证明上述假设的下一步是应用先定位后纯化的内源性受体作为糖组织化学标记物。必须指出的是，植物和动物凝集素的精细特异性可能不同，尽管它们具有相同的单糖特异性。因此，用于定位糖配体结合蛋白和内源性凝集素以检测合适的结合伙伴的新糖缀合物是有希望的探针，可以增强我们对细胞原位蛋白质-碳水化合物识别能力的了解。
Oligosaccharides can store biological information. In this respect, their capacity even outmatches that of oligo- and polymeric structures of nucleotides and amino acids, Protein-carbohydrate interactions are thus considered to be involved in the regulation of diverse cellular activities. Over decades, plant lectins have proven valuable for assessing structural aspects of the enormous variety of carbohydrate epitopes and for monitoring spatially and/or temporally restricted patterns of expression. If the presence of these epitopes and the alterations in their occurrence bear physiological relevance, one reasonable possibility is that the visualized saccharides serve as ligands in an operative protein-carbohydrate recognition system. To support the validity of this hypothesis, receptor sites for a sugar compound must be localized. Carrier-immobilized carbohydrates (neoglycocon-jugates) are adequate for this purpose. Chemical synthesis gains access to such probes. In the first stage, the presence of binding sites such as lectins in the tissue is ascertained. The next step toward proving the outlined hypothesis is the application of the first localized then purified endogenous receptors as glycohistochemical markers. It is essential to point out that the fine specificities of plant and animal lectins can differ, although they share an identical monosaccharide specificity. Thus, neoglycocon-jugates for localizing sugar ligand-binding proteins and endogenous lectins to detect suitable binding partners are promising probes to enhance our knowledge about the capacities of cells to be engaged in protein-carbohydrate recognition in situ.