Methanogenic population dynamics during startup of a full-scale anaerobic sequencing batch reactor treating swine waste
Methanogenic population dynamics during startup of a full-scale anaerobic sequencing batch reactor treating swine waste
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DOI：
10.1016/s0043-1354(02)00199-9
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发表时间：
2002-11-01
期刊：
影响因子：
12.8
通讯作者：
Raskin, L
中科院分区：
文献类型：
--
作者：
Angenent, LT;Sung, SW;Raskin, L
在以农场为基础的全规模厌氧序批式反应器(ASBR)启动期间，跟踪了产甲烷种群水平的变化，这些变化与操作和性能数据有关。ASBR接种来自城市污水处理设施的厌氧消化污泥。在大约3个月的驯化期内，ASBR的含量被稀释，以保持总氨氮水平约为2000 mg L(-1)。经过这段驯化期后，挥发性固体负荷增加到设计值1.7g L(-1)天(-1)，水力停留时间为15天，使ASBR中的总氨氮水平增加到约3600 mg L(-1)。在此期间，利用醋酸盐的甲烷产甲烷菌的16S核糖体RNA(16S RRNA)水平从3.8%下降到1.2%(占总16S rRNA水平的百分比)，而康氏甲烷藻的16S rRNA水平保持在较低水平(低于2.2%)。甲烷微生态菌目的产氢产甲烷菌的16S rRNA水平从2.3%增加到7.0%时，甲烷产量和反应器性能没有受到影响。因此，在高氨水平运行期间，甲烷产生的主要途径可能是乙酸酯氧化菌和利用氢气的产甲烷菌之间的同养关系。总氨氮水平超过3500 mg L(-1)的厌氧消化显然是可持续的，这是因为利用氢气的甲烷菌适应了高氨氮水平。(C)2002爱思唯尔科学有限公司。保留所有权利。
Changes in methanogenic population levels were followed during startup of a full-scale, farm-based anaerobic sequencing batch reactor (ASBR) and these changes were linked to operational and performance data. The ASBR was inoculated with anaerobic digester sludge from a municipal wastewater treatment facility. During an acclimation period of approximately 3 months, the ASBR content was diluted to maintain a total ammonia-N level of approximately 2000 mg l(-1). After this acclimation period, the volatile solids loading rate was increased to its design value of 1.7 g l(-1) day(-1) with a 15-day hydraulic retention time, which increased the total ammonia-N level in the ASBR to approximately 3600 mg l(-1). The 16S ribosomal RNA (rRNA) levels of the acetate-utilizing methanogens of the genus Methanosarcina decreased from 3.8% to 1.2% (expressed as a percentage of the total 16S rRNA levels) during this period, while the 16S rRNA levels of Methanosaeta concilii remained low (below 2.2%). Methane production and reactor performance were not affected as the 16S rRNA levels of the hydrogen-utilizing methanogens of the order Methanomicrobiales increased from 2.3% to 7.0%. Hence, it is likely that during operation with high ammonia levels, the major route of methane production is through a syntrophic relationship between acetate-oxidizing bacteria and hydrogen-utilizing methanogens. Anaerobic digestion at total ammonia-N levels exceeding 3500 mg l(-1) was sustainable apparently due to the acclimation of hydrogen-utilizing methanogens to high ammonia levels. (C) 2002 Elsevier Science Ltd. All rights reserved.