Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung
Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung
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DOI：
10.1007/978-1-61779-523-7_14
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Peng, Xu
中科院分区：
文献类型：
--
作者：
Jin, Yixin;Liu, Yang;Peng, Xu
血管网络的形成是脊椎动物胚胎发育的基础事件。血管生成和血管生成是血管形成的基本过程。血管内皮细胞在血管生成和血管生成过程中起着关键作用，培养的血管内皮细胞为探讨血管生成和血管生成的分子机制提供了不可或缺的模型。在这一章中，我们描述了一种使用PECAM-1包被的动态微球从小鼠心脏和肺中分离血管内皮细胞的方法。这种方法将提供多达10(7)个高纯度(>；85%)的内皮细胞。分离的内皮细胞保留了它们在体内的特性，如细胞表面标志PECAM-1和ICAM-2的表达。
The formation of blood vessel networks is a fundamental event in vertebrate embryo development. Angiogenesis and vasculogenesis are the essential processes in vascular formation. Endothelial cells play a key role during angiogenesis and vasculogenesis, and cultured vascular endothelial cells provide an indispensable model for exploring the molecular mechanisms of angiogenesis and vasculogenesis. In this chapter, we described a protocol using PECAM-1-coated Dynabeads for the isolation of vascular endothelial cells from mouse heart and lung. This method will provide up to 10(7) endothelial cells with high purity (>85%). The isolated endothelial cells retain their in vivo characteristics, such as the expression of the cell surface markers PECAM-1 and ICAM-2.