Sex differentiation of the gonad of fry transplanted into the anterior chamber of the adult eye in the teleost, Oryzias latipes.
Sex differentiation of the gonad of fry transplanted into the anterior chamber of the adult eye in the teleost, Oryzias latipes.
复制标题
移植到硬骨鱼成年眼前房的鱼苗性腺的性别分化。
作者：
N. Satoh
为了研究米达科鱼（Oryzias latipes）的性别分化是否受到成鱼性激素生理水平的影响，我们将含有新孵化鱼苗性腺的躯干区域单侧移植到成鱼眼睛的前房。根据血管形成情况可将移植物分为两种类型。一类接枝发育良好；其中一些从寄主鱼的眼睛里伸出来。在这些移植物中，移植物和宿主之间的血液循环连接无一例外地被检测到。另一种类型的移植物包括那些没有血管形成的标本。这种类型的移植物没有生长；这种情况似乎类似于所谓的啮齿动物眼睛前房的体内培养。然而，大多数移植物都退化了。从移植体中性腺的组织学来看，无论宿主的性别如何，鱼眼睛中的遗传雄性移植物都发育成睾丸。两个系列的实验证实了这一事实。遗传雌性鱼苗的性腺如果移植到雌性鱼体内，就会发育成卵巢。另一方面，将遗传雌性移植物的性腺移植到雄性鱼体内，不能发育成卵巢，而是在结构异常的性腺中形成了生精细胞。因此，雄鱼从遗传卵原细胞向生精细胞的性别分化逆转很可能是由生理水平的性激素完成的。相反，遗传雄性向雌性的性别逆转不是由雌性性激素的生理水平引起的。
In order to examine whether or not sex differentiation in the medaka, Oryzias latipes , is modified by the physiological level of sex hormones in the adult fish, trunk regions containing the gonads of newly hatched fry were transplanted unilaterally into the anterior chamber of the eyes of adult fish. The grafts could be classified into two types according to the vascularization. One type of graft developed well; some of them protruded from the eyes of the host fish. In these grafts the connexion of the blood circulation between the graft and the host was detectable without exception. The other type of graft consisted of those specimens without vascularization. The grafts of this type did not grow in size; this condition seemed to be similar to the so-called in vivo culture in the anterior chamber of the eye in rodents. Most of these grafts, however, degenerated. Judging from the histology of the gonad in the graft, a genetic male graft in the eye of a fish developed into a testis, regardless of the sexuality of the host. This fact was confirmed by two series of experiments. The gonad of a genetic female fry developed into an ovary if the graft was transplanted into a female fish. On the other hand, the gonad of a genetic female graft transplanted into a male fish failed to develop into an ovary, but formed spermatogenetic cells in a gonad of an abnormal structure. Therefore, it is highly probable that the reversal of sex differentiation from genetic oogonia into spermatogenetic cells is accomplished by the physiological level of the sex hormones in male fish. On the contrary, the sex reversal of genetic males to females is not induced by the physiological level of female sex hormones.