The catalytic center in nitrous oxide reductase, Cuz, is a copper-sulfide cluster
The catalytic center in nitrous oxide reductase, Cuz, is a copper-sulfide cluster
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DOI：
10.1021/bi001811i
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发表时间：
2000-10-24
期刊：
影响因子：
2.9
通讯作者：
Thomson, AJ
中科院分区：
文献类型：
--
作者：
Rasmussen, T;Berks, BC;Thomson, AJ
一氧化二氮还原酶是催化细菌反硝化最后一步的酶，其中一氧化二氮还原为二氮，其晶体结构表现出一个新的催化位点，称为 Cu-z。它包含由七个组氨酸和三个其他配体结合的四个铜离子簇，在 X 射线结构中被建模为 OH- 或 H2O。然而，同位素标记酶的元素分析和共振拉曼光谱最终证明 Cu-z 具有一种对酸不稳定的硫配体。因此，一氧化二氮还原酶含有第一个报道的生物硫化铜簇。
The crystal structure of nitrous oxide reductase, the enzyme catalyzing the final step of bacterial denitrification in which nitrous oxide is reduced to dinitrogen, exhibits a novel catalytic site, called Cu-z. This comprises a cluster of four copper ions bound by seven histidines and three other ligands modeled in the X-ray structure as OH- or H2O. However, elemental analyses and resonance Raman spectroscopy of isotopically labeled enzyme conclusively demonstrate that Cu-z has one acid-labile sulfur ligand. Thus, nitrous oxide reductase contains the first reported biological copper-sulfide cluster.