Evaluation of in situ albumin binding surfaces: a study of protein adsorption and platelet adhesion
Evaluation of in situ albumin binding surfaces: a study of protein adsorption and platelet adhesion
复制标题
DOI：
10.1007/s10856-010-4169-3
复制
发表时间：
2011-01-01
影响因子：
3.7
通讯作者：
Subramanian, Anuradha
中科院分区：
文献类型：
--
作者：
Thakurta, Sanjukta Guha;Subramanian, Anuradha
利用白蛋白的钝化作用的表面改性策略在生物材料表面的开发中是重要的。在这项研究中，线性肽（LP 1，LP 2）和一个小的化学配体（SCL）与白蛋白结合亲和力接枝到硅烷官能化的硅衬底。表面的接触角和椭圆偏振测量，其特征在于，和固定化配体的密度进行了光谱评估。椭圆偏振测量的厚度与接枝部分的预测分子长度相关。接触角分析表明，与SCL官能化和对照表面相比，LP 1和LP 2官能化表面是亲水性的。通过特异性酶联免疫吸附试验和2D凝胶电泳，对人血清中白蛋白的吸附进行了评价和定量。对于表面吸附的白蛋白，注意到以下趋势：LP 1> LP 2> SCL > C，其中LP 1衍生表面具有类似于2.450 μ g/cm（2）的结合白蛋白。与对照表面相比，LP 1衍生表面具有最少数量的吸附血小板，具有圆形血小板形态。
Surface modification strategies that take advantage of the passivation effects of albumin are important in the development of biomaterial surfaces. In this study, linear peptides (LP1, LP2) and a small chemical ligand (SCL) with albumin binding affinities were grafted onto silane functionalized silicon substrates. Surfaces were characterized with contact angle and ellipsometric measurements, and densities of immobilized ligands were assessed spectro-scopically. Ellipsometrically measured thickness correlated with the predicted molecular lengths of grafted moieties. Contact angle analysis indicated that the LP1 and LP2 functionalized surfaces were hydrophilic compared to SCL functionalized and control surfaces. Adsorption of albumin from human serum was evaluated and quantified via specific enzyme-linked immunosorbent assays and 2D gel electrophoresis. The following trend was noted for surface adsorbed albumin: LP1 > LP2 > SCL > C, with LP1 derivatized surfaces having similar to 2.450 mu g/cm(2) of bound albumin. LP1 derivatized surfaces possessed the least number of adsorbed platelets with rounded platelet morphology when compared to control surface.