Extraction, purification of Lycium barbarum polysaccharides and bioactivity of purified fraction
Extraction, purification of Lycium barbarum polysaccharides and bioactivity of purified fraction
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DOI：
10.1016/j.carbpol.2011.04.023
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发表时间：
2011-08
影响因子：
11.2
通讯作者：
Mang Ke;Xian-jun Zhang;Zihua Han;Hong-yuan Yu;Yung-Ta Lin;Wen-gang Zhang;F. Sun;Tian-ji Wang
中科院分区：
文献类型：
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作者：
Mang Ke;Xian-jun Zhang;Zihua Han;Hong-yuan Yu;Yung-Ta Lin;Wen-gang Zhang;F. Sun;Tian-ji Wang
从枸杞干果实中提取多糖。采用离子交换层析法对多糖进行分离纯化，得到了一种分子量为5.3 × 104Da的水溶性复合多糖LBPF 5。LBPF 5由阿拉伯糖、甘露糖、木糖、葡萄糖和鼠李糖组成。初步体外试验表明，该药物具有较强的清除自由基的活性。LBPF 5以浓度和时间依赖性方式抑制AAPH诱导的红细胞氧化溶血。LBPF 5和花青素对人膀胱癌细胞株BIU 87的生长有抑制作用，并能诱导BIU 87细胞凋亡。此外，花色苷具有较强的抗肿瘤活性。从这些结果，证明LBPF 5具有在体外和体内保护免受氧化应激损伤的能力。
Polysaccharides was extracted from driedLycium barbarumfruits. The ion-exchange chromatography was used to isolate and purify the polysaccharides to afford a complex water-soluble polysaccharide, named LBPF5 with a molecular mass of 5.3 × 104Da. LBPF5 consisted of arabose, mannose, xylose, glucose and rhamnose. Preliminary tests in vitro showed strong free radicals scavenging activities. The oxidative hemolysis of erythrocytes induced by AAPH was suppressed by the LBPF5 in a concentration- and time-dependent manner. In addition, LBPF5 and anthocyan could inhibit the growth of human bladder carcinoma cell line BIU87 and induce BIU87 apoptosis. Furthermore, anthocyan displayed stronger antitumor activities. From these results, it was demonstrated that LBPF5 has the ability to protect against damage from oxidative stress in vitro and in vivo.