Surface Topography Hinders Bacterial Surface Motility
Surface Topography Hinders Bacterial Surface Motility
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DOI：
10.1021/acsami.7b16715
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发表时间：
2018-03-21
影响因子：
9.5
通讯作者：
Ducker, William A.
中科院分区：
文献类型：
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作者：
Chang, Yow-Ren;Weeks, Eric R.;Ducker, William A.
我们证明，铜绿假单胞菌的表面运动受到波长在 2-8 μm 范围内的结晶半球形地形的阻碍。通过分析保持在 37°C 的流动生长培养基中的细胞的延时显微镜图像来确定运动性。5 分钟内细菌的净位移在包含 2-8 μm 半球的表面上比在平坦地形上低得多，但在 1 μm 半球上的位移并不低。也就是说，对于形貌的响应存在1到2μm之间的阈值。 4 μm 半球上的细胞比其他方向更有可能平行于局部晶轴行进。与平坦表面相比，8μm 地形上的细胞不太可能穿过半球的冠部，并且更有可能进行 30 度至 50 度的转动。这些结果表明，表面形貌可以作为表面运动的重要障碍，因此可能阻碍细菌的表面探索。由于表面探索可能是细菌形成菌落和寻求营养物质的过程的一部分，因此这些结果有助于阐明表面形貌阻碍生物膜形成的机制。
We demonstrate that the surface motility of the bacterium, Pseudomonas aeruginosa, is hindered by a crystalline hemispherical topography with wavelength in the range of 2-8 mu m. The motility was determined by the analysis of time-lapse microscopy images of cells in a flowing growth medium maintained at 37 degrees C. The net displacement of bacteria over 5 min is much lower on surfaces containing 2-8 mu m hemispheres than on flat topography, but displacement on the 1 mu m hemispheres is not lower. That is, there is a threshold between 1 and 2 mu m for response to the topography. Cells on the 4 mu m hemispheres were more likely to travel parallel to the local crystal axis than in other directions. Cells on the 8 mu m topography were less likely to travel across the crowns of the hemispheres and were also more likely to make 30 degrees-50 degrees turns than on flat surfaces. These results show that surface topography can act as a significant barrier to surface motility and may therefore hinder surface exploration by bacteria. Because surface exploration can be a part of the process whereby bacteria form colonies and seek nutrients, these results help to elucidate the mechanism by which surface topography hinders biofilm formation.