Unique molecular signatures of glycerophospholipid species in different rat tissues analyzed by tandem mass spectrometry
Unique molecular signatures of glycerophospholipid species in different rat tissues analyzed by tandem mass spectrometry
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DOI：
10.1016/j.bbalip.2006.05.010
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发表时间：
2006-09-01
影响因子：
4.8
通讯作者：
Cui, Zheng
中科院分区：
文献类型：
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作者：
Hicks, Amy M.;DeLong, Cynthia J.;Cui, Zheng
动物组织中的甘油磷脂（GPL）由大量的分子种类组成，这些分子种类主要在脂肪酰基组成上不同。为了进一步了解GPL在分子水平上的作用，有必要对动物组织中这些分子的分子组成进行全面，准确的描述。然而，这项任务之所以困难，仅仅是因为分析GPL物种的传统技术在很大程度上依赖于技术技能的准确性和可靠性，并且是极其劳动密集型的。近年来，串联质谱（MS/MS）被证明是一种高度可靠和灵敏的技术，用于分析生物样品中的小分子，包括GPL。在这项研究中，我们使用该技术进行同时比较分析的磷脂酰胆碱（PC），磷脂酰乙醇胺（PE），磷脂酰丝氨酸（PS）和磷脂酰肌醇（PI）在同一脂质制剂的肝，肺，肾，心脏，胰腺，胃，小肠，脾脏，骨骼肌和大脑的成年大鼠。我们在这10种不同的组织中产生了这4种GPL类别的分子谱，这些组织在同一样品的不同扫描之间以及来自不同动物的样品之间具有高度可重复性。令人感兴趣的是，发现每个组织都具有独特的GPL特征，可用于识别未知组织。更重要的是，这些图谱还可以为研究不同生理和病理条件下GPL代谢的变化提供参考点。(c)2006 Elsevier B. V.保留所有权利。
Glycerophospholipids (GPL) in animal tissues are composed of a large array of molecular species that mainly differ in the fatty acyl composition. In order to further understand the roles of GPL at the molecular level, it is necessary to have comprehensive, accurate accounts of the molecular makeup for these molecules in animal tissues. However, this task was difficult simply because the conventional technologies of profiling GPL species depended heavily on technical skill for accuracy and reliability and were extremely labor-intensive. In recent years, tandem mass spectrometry (MS/MS) proved to be a highly reliable and sensitive technology for profiling small molecules, including GPL, in biological samples. In this study, we used this technology to perform simultaneous comparative analyses for phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI) in the same lipid preparations of liver, lung, kidney, heart, pancreas, stomach, small intestine, spleen, skeleton muscle and brain of an adult rat. We produced molecular profiles of these 4 GPL classes in these 10 different tissues that are highly reproducible between different scans of the same sample and between samples from different animals. It is intriguing that each tissue was found to possess a unique signature of GPL profile that may be used to identify unknown tissues. More importantly, these profiles may also set reference points for studying changes of GPL metabolism in different physiological and pathological conditions. (c) 2006 Elsevier B.V. All rights reserved.