High expression of PCBP2 is associated with progression and poor prognosis in patients with glioblastoma
High expression of PCBP2 is associated with progression and poor prognosis in patients with glioblastoma
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DOI：
10.1016/j.biopha.2017.07.103
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发表时间：
2017-10-01
影响因子：
7.5
通讯作者：
Zhuang, Kai
中科院分区：
文献类型：
--
作者：
Luo, Kui;Zhuang, Kai
背景：聚（C）结合蛋白2（PCBP 2）已被发现在多种癌症中具有不明确的功能。然而，PCBP 2的具体生物学功能及其在胶质母细胞瘤中的作用机制尚不清楚。我们研究了143例胶质母细胞瘤标本中PCBP 2的表达，以探讨PCBP 2表达与临床病理参数之间的关系以及临床意义。方法：采用实时荧光定量PCR（qRT-PCR）和western blot方法检测143例胶质母细胞瘤患者PCBP 2的转录和翻译水平。结果：PCBP 2在高分期胶质母细胞瘤中的表达明显高于低分期胶质母细胞瘤（P < 0.001）; PCBP 2的高表达与临床分期和组织学分级有关（P < 0.001）。进一步的研究表明PCBP 2表达上调与胶质母细胞瘤患者预后不良有关（P < 0.001）。PCBP 2基因敲低可显著降低胶质母细胞瘤细胞殖民地形成能力和侵袭能力（P < 0.01）。结论：PCBP 2可能是胶质母细胞瘤的一个新的预后标志物和潜在的治疗靶点。(C)2017年由Elsevier Masson SAS出版。
Background: Poly(C)-binding protein 2 (PCBP2) has been found to have ambiguous functions in a variety of cancers. However, the specific biological function of PCBP2 and its mechanism in glioblastoma remain unclear. We investigated the expression of PCBP2 in 143 glioblastoma specimens to explore the linkage between PCBP2 expression and clinicopathological parameters as well as clinical significance. Furthermore, the underlying mechanisms of PCBP2 on glioblastoma progression were discussed in vitro.Methods: The transcriptional and translational levels of PCBP2 in 143 glioblastoma patients were detected by quantitative Real-time PCR (qRT-PCR) and western blot. The association of prognostic outcomes and PCBP2 expression was evaluated using Kaplan-Meier analysis.Results: PCBP2 expression was markedly increased in higher stages of glioblastoma compared with those in lower stages (P < 0.001). High expression of PCBP2 was associated with higher clinical stage and histological grade (P < 0.001). Further research suggested that PCBP2 upregulation was connected with poorer prognosis in patients with glioblastoma (P < 0.001). Moreover, PCBP2 knockdown could significantly decreased the colony formation and invasion capability of glioblastoma cells (P < 0.01). Conversely, PCBP2 overexpression could increase the colony formation and invasion capability (P < 0.01).Conclusion: These findings indicated that PCBP2 might be a novel prognostic biomarker and a potential therapeutic target of glioblastoma. (C) 2017 Published by Elsevier Masson SAS.