Antioxidant, Anti-inflammatory, and Antiproliferative Activities of Taxillus sutchuenensis
Antioxidant, Anti-inflammatory, and Antiproliferative Activities of Taxillus sutchuenensis
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DOI：
10.1142/s0192415x12500267
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发表时间：
2012-01-01
影响因子：
5.7
通讯作者：
Huang, Guan-Jhong
中科院分区：
文献类型：
--
作者：
Liu, Chia-Yu;Lin, Ying-Chih;Huang, Guan-Jhong
炎症与多种慢性疾病有关，包括癌症和动脉粥样硬化。苏川杉（Lecomte）丹参是台湾特有的民间药用植物。本研究的目的是评价水乙醇提取物（AETS）及其组分的抗氧化、抗炎和抗增殖活性。检测TEAC、DPPH自由基、总酚类化合物、总黄酮含量、抑制lps诱导的RAW264.7细胞NO生成及抑制癌细胞增殖。乙酸乙酯（EA）组分对TEAC和DPPH自由基的清除能力最强。EA部分的多酚和类黄酮含量也最高。EA组分还能降低lps诱导的RAW264.7细胞中NO的生成以及iNOS和COX-2的表达。采用体外培养A549细胞，研究了水/乙醇提取物及其组分的抗增殖活性，结果与它们的抗氧化能力一致。EA部位抗增殖活性最高，IC50为454.38 +/- 1.48 μ g/ml。槲皮素还具有抗氧化、抗炎和抗增殖活性。槲皮素可能是苏川赤霉素中一种重要的生物活性物质。实验结果表明，冬青是一种有效的抗氧化药用植物，其抗氧化作用可能与其多酚类化合物有关。
Inflammation is related to several chronic diseases, including cancer and atherosclerosis. Taxillus sutchuenensis (Lecomte) Danser is a special folk medicinal plant in Taiwan. The aim of this study was to evaluate the antioxidant, anti-inflammatory, and antiproliferative activities of the aqueouse-thanol extract from T. sutchuenensis (AETS) and its fractions. TEAC, DPPH radicals, total phenolic compounds, total flavonoid content, inhibition of NO production in LPS-induced RAW264.7 cells, and inhibition of cancer cell proliferation were tested. Among all fractions, the ethyl-acetate (EA) fraction showed the highest TEAC and DPPH radical scavenging activities. The EA fraction also had the highest polyphenol and flavonoid content. The EA fractions also decreased LPS-induced NO production and the expression of iNOS and COX-2 in RAW264.7 cells. The antiproliferative activities of the aqueous/ethanol extract and fractions were studied in vitro using A549 cells, and the results were consistent with their antioxidant capacities. EA fractions had the highest antiproliferative activity with an IC50 of 454.38 +/- 1.48 mu g/ml. Quercetin also had antioxidant, anti-inflammatory, and antiproliferative activities. Quercetin might be an important bioactive compound in T. sutchuenensis. The experimental data indicated that T. sutchuenensis is a potent antioxidant medicinal plant, and such efficacy may be mainly attributed to its polyphenolic compounds.