Sulfakinin inhibits activity of digestive enzymes in the brown planthopper, Nilaparvata lugens
Sulfakinin inhibits activity of digestive enzymes in the brown planthopper, Nilaparvata lugens
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Sulfakin 抑制褐飞虱 Nilaparvata lugens 消化酶的活性
DOI：
10.1016/j.aspen.2020.09.004
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发表时间：
2020-12-01
影响因子：
1.5
通讯作者：
Wu, Shun-Fan
中科院分区：
文献类型：
--
作者：
Guo, Di;Zhang, Su;Wu, Shun-Fan
在动物中，进食可以调节消化酶的释放。消化酶的产生和释放是对特定比例的营养素做出反应的，因此摄入食物的质量和数量是消化酶分泌和活性的重要因素。总体而言，进食昆虫的酶活性和分泌量相对高于未进食昆虫。神经肽和肽类激素是酶活性的重要调节因子。在一些昆虫中，已知神经肽磺胺激肽（SK）是摄食和消化的调节剂，类似于哺乳动物中的胆囊收缩素。然而，饲料和SK在重要害虫褐飞虱（Nilaparvata lugens）消化酶活性调节中的作用尚不清楚。在这项研究中，我们确定了六个基因编码不同的消化酶和克隆其中三个。我们发现，消化酶活性基因的酶活性和转录水平上调饥饿24 h后再喂动物5 h。此外，注射N.褐变SK降低消化酶活性并导致消化酶基因转录物的下调。本研究为半变态昆虫的食物和SK对消化酶的调节提供了新的视角。结合SK在诱导饱腹感中的作用，我们的数据有力地表明SK信号传导在调节食物摄取和加工中是重要的。
In animals, feeding can regulate release of digestive enzymes. Digestive enzymes are produced and released in response to specific ratios of nutrients, so the quality and quantity of food ingested are important factors in the secretion and activity of digestive enzymes. In general, the enzyme activity and secretion in the fed insects are relatively higher than that in the unfed insects. Neuropeptides and peptide hormones are important regulators of enzyme activity. In several insects, the neuropeptide sulfakinin (SK) is known to be a regulator of feeding and digestion similar to cholecystokinin in mammals. However, the roles of diet and SK in regulation of activity of digestive enzymes in the important pest insect, the brown planthopper (Nilaparvata lugens), are unknown. In this study, we identified six genes encoding different digestive enzymes and cloned three of these. We found that enzymatic activity and transcriptional levels of digestive enzymatic activity genes were upregulated by refeeding animals for 5 h after 24 h starvation. Furthermore, injection of N. lugens SK reduces digestive enzyme activity and leads to a downregulation of digestive enzyme gene transcripts. This study provides new views into the action of diet and SK in regulation of digestive enzymes in (hemimetabolous) insects. Taken together with the roles of SK in inducing satiety, our data strongly suggest that SK signaling is important in regulation of food ingestion and processing.