Quasi-Continuous Wave Near-Infrared Excitation of Upconversion Nanoparticles for Optogenetic Manipulation of C-elegans
Quasi-Continuous Wave Near-Infrared Excitation of Upconversion Nanoparticles for Optogenetic Manipulation of C-elegans
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DOI：
10.1002/smll.201503792
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发表时间：
2016-04-06
期刊：
影响因子：
13.3
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Bansal, Akshaya;Liu, Haichun;Zhang, Yong
光遗传学是一种新兴的强大工具来研究神经系统的运作。然而，低组织穿透可见光的使用限制了其治疗潜力。采用深穿透近红外（NIR）光用于光遗传学将是有益的，但它不能直接使用。这个问题可以用上转换纳米颗粒（UCN）来解决，所述上转换纳米颗粒充当纳米换能器，在NIR光激发时以延伸到UV范围的较短波长发射。虽然有吸引力，但这种NIR光遗传学的实施受到低UCN发射强度的阻碍，低UCN发射强度需要高NIR激发强度，导致过热问题。开发了一种新的准连续波（准CW）激发方法，该方法显着增强了UCN的多光子发射，并首次在体外和C.优美的这里开发的方法使得能够以显著较低的激发功率和沿着的UCN浓度激活通道视紫红质-2，如在CW激发下所见，具有可忽略的光毒性，为治疗性光遗传学铺平了道路。
Optogenetics is an emerging powerful tool to investigate workings of the nervous system. However, the use of low tissue penetrating visible light limits its therapeutic potential. Employing deep penetrating near-infrared (NIR) light for optogenetics would be beneficial but it cannot be used directly. This issue can be tackled with upconversion nanoparticles (UCNs) acting as nanotransducers emitting at shorter wavelengths extending to the UV range upon NIR light excitation. Although attractive, implementation of such NIR-optogenetics is hindered by the low UCN emission intensity that necessitates high NIR excitation intensities, resulting in overheating issues. A novel quasi-continuous wave (quasi-CW) excitation approach is developed that significantly enhances multiphoton emissions from UCNs, and for the first time NIR light-triggered optogenetic manipulations are implemented in vitro and in C. elegans. The approach developed here enables the activation of channelrhodopsin-2 with a significantly lower excitation power and UCN concentration along with negligible phototoxicity as seen with CW excitation, paving the way for therapeutic optogenetics.