FBXW7 Mutations Promote Cell Proliferation, Migration, and Invasion in Cervical Cancer
FBXW7 Mutations Promote Cell Proliferation, Migration, and Invasion in Cervical Cancer
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FBXW7 突变促进宫颈癌细胞增殖、迁移和侵袭。
DOI：
10.1089/gtmb.2018.0278
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发表时间：
2019-06-01
影响因子：
1.4
通讯作者：
Huang, Ouping
中科院分区：
文献类型：
--
作者：
Liu, Faying;Zou, Yang;Huang, Ouping
目的：宫颈癌是最常见的妇科癌症。最近的研究表明，含有编码Skp1-Cul1-F-box蛋白（SCF）泛素连接酶亚基的F-box和WD重复结构域7 （FBXW7）基因在宫颈鳞状细胞癌中经常发生突变。在这项研究中，我们研究了中国宫颈癌细胞是否也含有这些突变。方法：采用PCR和测序方法，对190例汉族宫颈癌患者标本进行FBXW7突变分析。结果：在145例（1.4%）宫颈鳞状细胞癌的研究中发现了两种FBXW7突变（p.R479P和p.L443H）。p.L443H体细胞突变以前没有报道过。功能分析表明，这两种FBXW7突变都能促进细胞增殖、迁移和侵袭。结论：FBXW7基因突变在宫颈鳞状细胞癌中占1.4%。然而，我们确实发现了一种新的FBXW7突变。我们的研究结果还表明，FBXW7突变可以促进宫颈癌细胞的增殖、迁移和侵袭。
Aim: Cervical cancer is the most common gynecological cancer. Recent studies have revealed that the F-box and WD repeat domain containing 7 (FBXW7) gene, which encodes a subunit of Skp1-Cul1-F-box protein (SCF) ubiquitin ligase, is frequently mutated in cervical squamous cell carcinomas. In this study, we investigated whether Chinese cervical cancer cells also harbor these mutations. Methods: Using PCR and sequencing assays, a total of 190 specimens from Han Chinese patients with cervical cancer were analyzed for FBXW7 mutations. Results: Two FBXW7 mutations (p.R479P and p.L443H), were identified from a study of 145 (1.4%) cervical squamous cell carcinomas. The p.L443H somatic mutation has not been previously reported. Functional assays showed that both of these FBXW7 mutations could promote cell proliferation, migration, and invasion. Conclusion: A low frequency (1.4%) of cervical squamous cell carcinomas were identified with FBXW7 mutations. We did, however, identify a novel FBXW7 mutation. Our results also demonstrated that the identified FBXW7 mutations could promote cell proliferation, migration, and invasion in cervical cancer cells.