Differences in susceptibility of Duroc, Hampshire, and Meishan pigs to infection with a high virulence strain (VR2385) of porcine reproductive and respiratory syndrome virus (PRRSV)
Differences in susceptibility of Duroc, Hampshire, and Meishan pigs to infection with a high virulence strain (VR2385) of porcine reproductive and respiratory syndrome virus (PRRSV)
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DOI：
10.1111/j.1439-0388.1998.tb00341.x
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发表时间：
1998-06-01
期刊：
影响因子：
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通讯作者：
Bruna, JD
中科院分区：
文献类型：
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作者：
Halbur, PG;Rothschild, MF;Bruna, JD
本研究的目的是确定对prrsv诱导的疾病和病变的抗性或易感性是否存在遗传影响。每窝2 ~ 3窝的3个品种的猪随机分为治疗组和对照组。处理方法是接种一种特征明确的高毒力PRRSV毒株（VR2385）。杜洛克、汉普郡和梅山猪在9-10日龄断奶，22-38日龄接种PRRSV。根据批准的动物使用和护理标准饲养和照顾动物。猪鼻内接种含有10(5.8)S TCID50 PRRSV VR2385的细胞培养基5 ml。对照组是模拟感染。每日记录临床疾病评分和直肠温度。接种后10天尸检时估计肉眼病变评分。所有器官系统均采用盲法显微镜检查。采用间接免疫荧光法测定血清抗体反应。汉普郡猪的宏观肺损伤评分（43.0 +/- 3.7）明显高于杜洛克猪（29.43 +/- 3.3）或梅山猪（25.0 +/- 3.5）。梅山猪肺中PRRSV抗原检出明显减少。梅山地区患者心肌炎、脑炎发生率明显高于其他地区。杜洛克人的PRRSV标准化血清抗体滴度明显降低。猪PRRSV诱发病变的严重程度和分布差异，以及标准化血清PRRSV抗体反应的差异，可能部分受到猪遗传因素的影响。选择对PRRSV感染的破坏性后果不太敏感的动物的能力将对养猪业具有巨大价值。
The objective of this research was to determine if there is genetic influence on the resistance or susceptibility to PRRSV-induced disease and lesions. Pigs of three breeds from two or three litters per ere randomly assigned to treatment or control groups. Treatment consisted of inoculation with a well characterized, high virulence strain (VR2385) of PRRSV. Duroc, Hampshire, and Meishan pigs were weaned at 9-10 days of age and inoculated with PRRSV at 22-38 days of age. Animals were housed and cared for according to approved animal use and care standards. Pigs were inoculated intranasally with 5 ml of cell culture media containing 10(5.8) S TCID50 of PRRSV VR2385. Controls were mock-infected. Clinical disease scores and rectal temperatures were recorded daily. Macroscopic lesion scores were estimated at necropsy at 10 days post inoculation. All organ systems were examined microscopically in a blinded fashion. Serum antibody response was measured by an indirect immunofluorescence assay. Hampshire pigs had significantly more severe macroscopic lung lesion scores (43.0 +/- 3.7) than Duroc (29.43 +/- 3.3) or Meishan (25.0 +/- 3.5) plgs. Meishan pigs had significantly less PRRSV antigen detected in the lungs. Significantly more Meishan plgs had myocarditis and encephalitis. Duroc plgs had significantly lower normalized serum antibody titres to PRRSV. Differences in severity and distribution of PRRSV-induced lesions, and in normalized serum antibody response to PRRSV may be in part influenced by pig genetic factors. The ability to select animals which are less susceptible to the devastating consequences of PRRSV infection would be of tremendous value to the swine industry.