Comparison of peptide enzyme-linked immunosorbent assay and radioimmunoprecipitation assay with in vitro-translated proteins for detection of serum antibodies to human papillomavirus type 16 E6 and E7 proteins.
Comparison of peptide enzyme-linked immunosorbent assay and radioimmunoprecipitation assay with in vitro-translated proteins for detection of serum antibodies to human papillomavirus type 16 E6 and E7 proteins.
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肽酶联免疫吸附测定和体外翻译蛋白放射免疫沉淀测定检测人乳头瘤病毒 16 型 E6 和 E7 蛋白血清抗体的比较。
DOI：
10.1128/jcm.32.9.2216-2220.1994
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发表时间：
1994
影响因子：
9.4
通讯作者：
Viscidi,RP
中科院分区：
文献类型：
--
作者：
Sun,Y;Shah,KV;Müller,M;Muñoz,N;Bosch,XF;Viscidi,RP
血清中抗人乳头瘤病毒(HPV)16型(HPV-16)E6和E7蛋白抗体是HPV相关性浸润性宫颈癌的标志物。我们比较了体外翻译的HPV-16E6和E7蛋白的放射免疫沉淀法和E6和E7合成肽的酶联免疫吸附试验两种方法在血清学上区分浸润性宫颈癌和正常对照的能力。在患者中，E6放射免疫沉淀法的抗体阳性率(55.7%)高于E6多肽EL ISA法(15.5%)，而在正常对照中，E6多肽EL ISA法的抗体阳性率(5%)低于放射免疫沉淀法(1.7%)。E7患者E7抗体阳性率放射免疫沉淀法(43%)与多肽ELISA法(41%)相当，但对照组E7多肽ELISA法(17.4%)高于放射免疫沉淀法(4.1%)。E7放射免疫沉淀法和E7多肽酶联免疫吸附试验在患者之间有很好的一致性，但在对照组中则不符合。在与代表性血清的测试中，翻译蛋白的热变性导致对E6蛋白的反应性完全丧失，对E7蛋白的反应性显著降低。我们的研究表明，放射免疫沉淀法对浸润性宫颈癌患者和对照组的鉴别能力优于多肽ELISA，这与放射免疫沉淀法检测构象表位的能力有关。
Antibodies to human papilloma virus (HPV) type 16 (HPV-16) E6 and E7 proteins in serum are markers for HPV-associated invasive cervical carcinoma. We compared two assays, a radioimmunoprecipitation assay with in vitro-translated HPV-16 E6 and E7 proteins and an enzyme-linked immunosorbent assay (ELISA) with E6 and E7 synthetic peptides, for their abilities to discriminate serologically between patients with invasive cervical cancer and controls. Among the patients, antibody prevalences were higher by the E6 radioimmunoprecipitation assay (55.7%) than by the E6 peptide ELISA (15.5%), but among the controls, they were lower by the radioimmunoprecipitation assay (1.7%) than by the E6 peptide ELISA (5%). For E7, antibody prevalences among the patients were comparable by the radioimmunoprecipitation assay (43%) and the peptide ELISA (41%), but among the controls they were higher by the E7 peptide ELISA (17.4%) than by the radioimmunoprecipitation assay (4.1%). There was good agreement between the E7 radioimmunoprecipitation assay and the E7 peptide ELISA among patients but not among controls. In tests with representative sera, heat denaturation of the translated proteins resulted in a complete loss of reactivity to the E6 protein and a marked decrease in reactivity to the E7 protein. Our study showed that the radioimmunoprecipitation assay discriminates better than the peptide ELISA between patients with invasive cervical cancer and controls and that this is related to the ability of the radioimmunoprecipitation assay to detect conformational epitopes.