Externally imposed electric field enhances plant root tip regeneration.
Externally imposed electric field enhances plant root tip regeneration.
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DOI：
10.1002/reg2.59
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Sena, Giovanni
中科院分区：
文献类型：
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作者：
Kral, Nicolas;Hanna Ougolnikova, Alexandra;Sena, Giovanni
在植物中，芽和根的再生可以在组织培养的独特条件下（体外）诱导，但在从组织损伤中恢复的完整个体（植物中）中也可以观察到。例如，根可以在植物中再生完全切除的分生组织，甚至在顶端干细胞生态位受损的突变体中也是如此。不幸的是，迄今为止，仍然缺乏对植物再生的全面了解。在这里，我们提供的证据表明，施加的电场会扰乱拟南芥根尖的再生。至关重要的是，我们通过分别改变切割的位置以及切除和刺激之间的时间间隔来探索残肢响应电刺激的空间和时间能力。我们的数据表明，平行于根部的电场的短暂脉冲足以将其再生的可能性增加两倍，并扰乱激素生长素的局部分布以及细胞分裂调节。值得注意的是，根部朝向阳极或阴极的方向被证明发挥了作用。
In plants, shoot and root regeneration can be induced in the distinctive conditions of tissue culture (in vitro) but is also observed in intact individuals (in planta) recovering from tissue damage. Roots, for example, can regenerate their fully excised meristems in planta, even in mutants with impaired apical stem cell niches. Unfortunately, to date a comprehensive understanding of regeneration in plants is still missing. Here, we provide evidence that an imposed electric field can perturb apical root regeneration in Arabidopsis. Crucially, we explored both spatial and temporal competences of the stump to respond to electrical stimulation, by varying respectively the position of the cut and the time interval between excision and stimulation. Our data indicate that a brief pulse of an electric field parallel to the root is sufficient to increase by up to two‐fold the probability of its regeneration, and to perturb the local distribution of the hormone auxin, as well as cell division regulation. Remarkably, the orientation of the root towards the anode or the cathode is shown to play a role.