Efficient synthesis of antisense phosphorothioate oligonucleotides: Evaluation of dichloroacetic acid at higher concentration to reduce cycle time
Efficient synthesis of antisense phosphorothioate oligonucleotides: Evaluation of dichloroacetic acid at higher concentration to reduce cycle time
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DOI：
10.1021/op030006j
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发表时间：
2003-11-01
影响因子：
3.4
通讯作者：
Ravikumar, VT
中科院分区：
文献类型：
--
作者：
Cheruvallath, ZS;Carty, RL;Ravikumar, VT
在脱三烷基化过程中，将甲苯中二氯乙酸的浓度从3% （v/v）提高到10%，可以提高硫代磷寡核苷酸的合成效率。在相同浓度下，二氯乙酸的效果优于二氯丙酸。
Efficiency of phosphorothioate oligonucleotide syntheses could be improved by increasing the concentration of dichloroacetic acid in toluene to 10% from 3% (v/v) during the detritylation step. It was also found that dichloroacetic acid is better than dichloropropionic acid when compared at similar concentrations.