Antibiotics, arsenate and H2O2 induce the promoter of Staphylococcus aureus cspC gene more strongly than cold
Antibiotics, arsenate and H2O2 induce the promoter of Staphylococcus aureus cspC gene more strongly than cold
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DOI：
10.1002/jobm.200800065
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发表时间：
2009-04-01
影响因子：
3.1
通讯作者：
Sau, Subrata
中科院分区：
文献类型：
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作者：
Chanda, Palas Kumar;Mondal, Rajkrishna;Sau, Subrata
细菌冷休克基因表达的蛋白质在序列水平上高度保守，在冷应激和正常细胞中具有多种生物学功能。为了研究不同药物对金黄色葡萄球菌冷休克基因的影响，我们克隆了金黄色葡萄球菌cspC基因的上游区域。金黄色葡萄球菌纽曼，并发现上述区域甚至在37 ℃下也具有明显的启动子（P-c）活性。记者S。金黄色葡萄球菌菌株CHANDA 2，通过将P-c-lacZ转录融合体插入到S.金黄色葡萄球菌RN 4220基因组中表达的β-半乳糖苷酶在冷休克后表达水平非常低，表明低温诱导P-c非常弱。有趣的是，P-c的转录被几种抗生素、过氧化氢和砷酸盐非常强烈地诱导。表达的冷休克蛋白。金黄色葡萄球菌在序列水平上高度相同，并带有单链核酸结合基序。在csp转录物的起始密码子下游和核糖体结合位点上游分别鉴定出一个16 nt的下游盒和一个13 nt的上游盒。他们在S.金黄色葡萄球菌冷休克基因的表达已被详细讨论。
Proteins expressed by the bacterial cold shock genes are highly conserved at sequence level and perform various biological functions in both the cold-stressed and normal cells. To study the effects of various agents on the cold shock genes of Staphylococcus aureus, we have cloned the upstream region of cspC from S. aureus Newman and found that the above region possesses appreciable promoter (P-c) activity even at 37 degrees C. A reporter S. aureus strain CHANDA2, constructed by inserting the P-c-lacZ transcriptional fusion into S. aureus RN4220 genome, was found to express very low level of beta-galactosidase after cold shock, indicating that low temperature induces P-c very weakly. Interestingly, transcription from P-c was induced very strongly by several antibiotics, hydrogen peroxide and arsenate salt. Cold shock proteins expressed by S. aureus are highly identical at sequence level and bear single-strand nucleic acid binding motifs. A 16 nt downstream box and a 13 nt upstream box were identified at the downstream of initiation codon and at the upstream of ribosome binding site of csp transcripts. Their roles in S. aureus cold shock gene expression have been discussed elaborately.