ETHYLENE-INDEPENDENT AND ETHYLENE-DEPENDENT BIOCHEMICAL-CHANGES IN RIPENING TOMATOES
ETHYLENE-INDEPENDENT AND ETHYLENE-DEPENDENT BIOCHEMICAL-CHANGES IN RIPENING TOMATOES
复制标题
DOI：
10.1104/pp.74.1.32
复制
发表时间：
1984-01-01
期刊：
影响因子：
7.4
通讯作者：
GRIERSON, D
中科院分区：
文献类型：
--
作者：
JEFFERY, D;SMITH, C;GRIERSON, D
L的果实。番茄Sonatine在6%CO2、6%O2和88%N2中于12 ℃储存14周。C，表现出与成熟相关的某些生化事件的时间分离。2柠檬酸循环酶，柠檬酸合成酶和苹果酸脱氢酶的比活性，大幅下降，在第一个2周的存储时，也发生了有机酸浓度的变化。在此期间，番茄红素，多聚半乳糖醛酸酶和乙烯检测不到。当果实从贮藏中取出时，乙烯被释放，多聚半乳糖醛酸酶和转化酶的活性迅速启动，番茄红素的合成也是如此。为了确定有机酸代谢的变化是否受到乙烯的影响，将果实保持在22 ℃。在正常气氛或补充有27 μ l/l乙烯的正常气氛中，在两种气氛中，在转化酶和多聚半乳糖醛酸酶的比活性的任何可检测的增加之前，柠檬酸循环酶的比活性发生了与上述类似的定量变化。后者的变化，连同色素的变化，发生在2和3天前，在水果暴露于乙烯，与那些保持在正常的气氛。
Fruits of L. esculentum Mill cv. Sonatine stored in 6% CO2, 6% O2, and 88% N2 for 14 wk at 12.degree. C, exhibited a temporal separation of certain biochemical events associated with ripening. The specific activity of 2 citric acid cycle enzymes, citrate synthase and malate dehydrogenase, fell substantially during the first 2 wk of storage when changes in organic acid concentration also occurred. During this period, lycopene, polygalacturonase and ethylene were undetectable. When fruits were removed from storage, ethylene was evolved and polygalacturonase and invertase activity were rapidly initiated as was synthesis of lycopene. To determine whether the changes in organic acid metabolism were affected by ethylene, fruit was kept at 22.degree. C in either a normal atmosphere or a normal atmosphere supplemented with 27 .mu.l/l of ethylene, and it was shown that in both atmospheres similar quantitative changes to those described above occurred in the citric acid cycle enzymes specific activities before any detectable increase in the specific activities of invertase and polygalacturonase. These latter changes, together with pigment changes, occurred between 2 and 3 days earlier in fruit exposed to ethylene, compared with those kept in a normal atmosphere.