Construction of recombinant swinepox viruses and expression of the classical swine fever virus E2 protein
Construction of recombinant swinepox viruses and expression of the classical swine fever virus E2 protein
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DOI：
10.1016/s0166-0934(00)00296-2
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发表时间：
2001-04-01
影响因子：
3.1
通讯作者：
Ahn, BY
中科院分区：
文献类型：
--
作者：
Hahn, J;Park, SH;Ahn, BY
探索猪痘病毒作为免疫活载体的可能性。开发了用于构建携带外源基因的重组SPV的载体。在该系统中，将外源基因置于痘苗病毒强启动子P-11下，插入病毒胸苷激酶（TK）基因中，通过与E. colilacZ基因。与野生型病毒相比，TK重组SPV在猪细胞中显示出适度的减毒水平，而在猴或人细胞中观察到更多的减毒。使用该系统，重组病毒表达的E？制备了猪瘟病毒（CSFV）糖蛋白。用伪狂犬病病毒的gX信号序列和E2的跨膜结构域进行工程改造，E2蛋白在感染细胞的细胞质中以二聚体形式表达。(C)2001 Elsevier Science B. V.保留所有权利。
To explore the swinepox virus (SPV) as a potential live vector for immunization. a vector was developed for the construction of a recombinant SPV carrying foreign genes. In this system, a foreign gene placed under the strong vaccinia virus promoter P-11 can be inserted into the viral thymidine kinase (TK) gene, and the recombinant virus can be isolated in a non-selective medium by the co-expression of E. coli lacZ gene. Compared with the wild type virus, the TK-recombinant SPV showed a modest level of attenuation in porcine cells while more attenuation was observed in monkey or human cells. Using this system, a recombinant virus expressing the E? glycoprotein of classical swine fever virus (CSFV) was produced. Engineered with the gX signal sequence of the pseudorabies virus, and transmembrane domain of E2, the E2 protein was expressed as a dimeric form in the cytoplasm of the infected cells. (C) 2001 Elsevier Science B.V. All rights reserved.