Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.)
Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.)
复制标题
DOI：
10.1016/s2095-3119(13)60575-4
复制
发表时间：
2014-01-01
影响因子：
4.8
通讯作者：
Li Zhi-kang
中科院分区：
文献类型：
--
作者：
Chai Lu;Zhang Jian;Li Zhi-kang
盐度是限制亚洲沿海地区水稻产量的主要因素。为选育耐盐水稻品种，利用SSR标记、AB-QTL分析和选择渐渗等方法，对2套BC 2F 5渐渗系在严重自然盐胁迫和非胁迫条件下4个产量性状的表型进行了分析。在盐胁迫和非盐胁迫条件下，对水稻4个产量性状有显著影响的QTLS均被鉴定出来，其中大部分（>90%）聚集在水稻基因组的13个基因组区域内，且存在复杂的上位性。盐胁迫和非盐胁迫条件下，多数影响产量性状的QTL均表现出差异表达。我们的研究结果表明，遗传学互补性提供了一个充分的解释隐藏的遗传多样性，ST在两个IL人群中观察到。本研究筛选出了在盐胁迫和非盐胁迫条件下均能显著提高产量性状、在多个QTL上具有良好供体等位基因的黄花占（HHZ）离子导入系，为利用分子标记辅助选择（MAS）或基因组选择改良水稻耐盐性提供了优良材料和相关遗传信息。
Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the whole-plant growth duration salt tolerance (ST) was genetically dissected by phenotyping two sets of BC2F5 introgression lines (ILs) for four yield traits under severe natural salt stress and non-stress filed conditions using SSR markers and the methods of advanced backcross QTL (AB-QTL) analysis and selective introgression. Many QTLS affecting four yield traits under salt stress and non-stress conditions were identified, most (>90%) of which were clustered in 13 genomic regions of the rice genome and involved in complex epistasis. Most QTLs affecting yield traits were differentially expressed under salt stress and non-stress conditions. Our results suggested that genetics complementarily provides an adequate explanation for the hidden genetic diversity for ST observed in both IL populations. Some promising Huanghuazhan (HHZ) ILs with favorable donor alleles at multiple QTLs and significantly improved yield traits under salt stress and non-stress conditions were identified, providing excellent materials and relevant genetic information for improving rice ST by marker-assisted selection (MAS) or genome selection.