Aggregation of Individual Sensing Units for Signal Accumulation: Conversion of Liquid-Phase Colorimetric Assay into Enhanced Surface-Tethered Electrochemical Analysis
Aggregation of Individual Sensing Units for Signal Accumulation: Conversion of Liquid-Phase Colorimetric Assay into Enhanced Surface-Tethered Electrochemical Analysis
复制标题
聚合各个传感单元以进行信号积累：将液相比色测定转换为增强的表面束缚电化学分析
DOI：
10.1021/jacs.5b04348
复制
发表时间：
2015-07-22
影响因子：
15
通讯作者：
Dai, Zhihui
中科院分区：
文献类型：
--
作者：
Wei, Tianxiang;Dong, Tingting;Dai, Zhihui
提出了一种新的概念，转换，液相比色分析到增强的表面拴系电化学分析，这是基于分析诱导的金属纳米粒子（MN）的网络结构的形成。在概念验证试验中，胸腺嘧啶功能化的银纳米粒子（Ag-T）被设计为用于Hg 2+测定的传感单元。通过T-Hg 2 +-T的特异性配位，基于功能化传感单元的验证系统不仅可以在比色法中表现良好，而且可以开发成更灵敏、更稳定的电化学Hg 2+传感器。在电化学分析中，分析物诱导MN聚集的简单原理可以用作双重信号放大策略，用于显著提高检测灵敏度。更重要的是，那些依赖于目标诱导的MN聚集的众多且不同的比色测定可以通过经由这种方法将它们转换成电化学测定来增强以满足灵敏分析的雄心勃勃的需求。
A novel concept is proposed for converting, liquid-phase colorimetric assay into enhanced surface-tethered electrochemical analysis, which is based on the analyte-induced formation of a network architecture of metal narioparticles (MNs). In a proof-of-concept trial, thymine-functionalized silver nanoparticle (Ag-T) is designed as the sensing unit for Hg2+ determination. Through a specific T-Hg2+-T coordination, the validation system based on functionalized sensing units not only can perform well in a colorimetric Hg2+ assay, but also can be developed into a more sensitive and stable electrochemical Hg2+ sensor. In electrochemical analysis, the simple principle Of analyte-induced aggregation Of MNs can be used as a dual signal amplification strategy for significantly improving the detection sensitivity. More importantly, those numerous and diverse colorimetric assays that rely on the target-induced aggregation of MNs an be augmented to satisfy the ambitious demands of sensitive analysis by converting them into electrochemical assays via this approach.