Age-related effect of aerobic exercise training on antioxidant and oxidative markers in the liver challenged by doxorubicin in rats
Age-related effect of aerobic exercise training on antioxidant and oxidative markers in the liver challenged by doxorubicin in rats
复制标题
DOI：
10.1080/10715762.2018.1470328
复制
发表时间：
2018-01-01
影响因子：
3.3
通讯作者：
Leicht, Anthony S.
中科院分区：
文献类型：
--
作者：
Ahmadian, Mehdi;Roshan, Valiollah Dabidi;Leicht, Anthony S.
本研究的目的是调查的氧化和抗氧化状态的肝组织挑战阿霉素和检查可能的保护作用的有氧运动对阿霉素诱导的氧化应激。将72只大鼠分为3个年龄组（年轻、成年和老年），3个给药亚组由每个年龄组8只大鼠组成：多柔比星、有氧运动+多柔比星和有氧运动+盐水。实验组进行常规跑步机跑步3周。多柔比星以20 mg/kg的剂量通过腹腔注射给药，而有氧运动+生理盐水组接受相当体积的生理盐水。热休克蛋白70，丙二醛，谷胱甘肽过氧化物酶，和蛋白质羰基测定干预期后的肝匀浆。与老年组相比，年轻组的氧化应激值较低，在所有组中，多柔比星治疗诱导肝毒性。包括有氧运动训练显着增加热休克蛋白70和抗氧化酶水平（谷胱甘肽过氧化物酶），而它降低氧化应激生物标志物（丙二醛和蛋白质羰基）的所有年龄组。这些结果表明，有氧运动训练可能是一种潜在的，非药物的策略，通过其对抗氧化剂水平和氧化应激生物标志物的积极影响来调节阿霉素诱导的肝毒性。
The aims of the current study were to investigate the oxidant and antioxidant status of liver tissue challenged by doxorubicin and to examine the possible protective effects of aerobic exercise on doxorubicin-induced oxidative stress. Seventy-two rats were divided into three age groups (Young, Adult, and Elderly) with three treatment subgroups consisting of eight rats per age group: doxorubicin, aerobic exercise + doxorubicin, and aerobic exercise + saline. The experimental groups performed regular treadmill running for 3 weeks. Doxorubicin was administered by i.p. injection at a dosage of 20 mg kg(-1 )while the aerobic exercise + saline group received saline of a comparable volume. Heat shock protein 70, malondialdehyde, glutathione peroxidase, and protein carbonyl were determined from the liver homogenates following the intervention period. Treatment with doxorubicin induced hepatotoxicity in all groups with lower values of oxidative stress in young compared with the older groups. The inclusion of aerobic exercise training significantly increased heat shock protein 70 and antioxidant enzyme levels (glutathione peroxidase) whereas it decreased oxidative stress biomarkers (malondialdehyde and protein carbonyl) for all age groups. These results suggest that aerobic exercise training may be a potential, non-drug strategy to modulate doxorubicin-induced hepatotoxicity through its positive impact on antioxidant levels and oxidative stress biomarkers.