A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS
A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS
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DOI：
10.1126/science.227.4691.1229
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
FRALEY, RT
中科院分区：
文献类型：
--
作者：
HORSCH, RB;FRY, JE;FRALEY, RT
通过一种新的叶盘转化-再生方法已经产生了转化的矮牵牛、烟草和番茄植物。用含有修饰的肿瘤诱导质粒（其中来自转移DNA的植物激素生物合成基因已被删除并被卡那霉素抗性嵌合基因取代）的根癌土壤杆菌菌株接种表面灭菌的叶盘并培养2天。然后将叶盘转移到含有卡那霉素的选择性培养基中。芽再生发生在2-4周内，转化体通过它们在含有卡那霉素的培养基中形成根的能力来确认。这种生产转化植物的方法将基因转移、植物再生和转化体的有效选择结合到一个过程中，并且应该适用于可以被农杆菌感染并从叶外植体再生的植物物种。
Transformed petunia, tobacco and tomato plants have been produced by means of a novel leaf disk transformation-regeneration method. Surface-sterilized leaf disks were inoculated with an Agrobacterium tumefaciens strain containing a modified tumor-inducing plasmid (in which the phytohormone biosynthetic genes from transferred DNA had been deleted and replaced with a chimeric gene for kanamycin resistance) and cultured for 2 days. The leaf disks were then transferred to selective medium containing kanamycin. Shoot regeneration occurred within 2-4 wk, and transformants were confirmed by their ability to form roots in medium containing kanamycin. This method for producing transformed plants combines gene transfer, plant regeneration and effective selection for transformants into a single process and should be applicable to plant species that can be infected by Agrobacterium and regenerated from leaf explants.