Rapid uncoupling of oxidative phosphorylation accompanies glutamate toxicity in rat cerebellar granule cells
Rapid uncoupling of oxidative phosphorylation accompanies glutamate toxicity in rat cerebellar granule cells
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DOI：
10.1097/00001756-199611040-00023
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发表时间：
1996-11-04
期刊：
影响因子：
1.7
通讯作者：
Calissano, P
中科院分区：
文献类型：
--
作者：
Atlante, A;Gagliardi, S;Calissano, P
A 100 μ M谷氨酸脉冲给药大鼠小脑颗粒细胞引起一个非常快速和渐进的减少，分别由葡萄糖和琥珀酸的添加引起的细胞和线粒体耗氧量。反映线粒体产生ATP的能力的呼吸控制率在添加谷氨酸后的前30分钟内降低了50%。谷氨酸暴露后，呼吸控制率在随后的3-5小时内逐渐下降至接近1。额外的钙的添加本身对细胞匀浆的氧消耗没有影响。
A 100 mu M glutamate pulse administered to rat cerebellar granule cells causes a very rapid and progressive decrease in both cell and mitochondrial oxygen consumption caused by glucose and succinate addition, respectively. The respiratory control ratio, which reflects the ability of mitochondria to produce ATP, is reduced by 50% within the first 30 min after glutamate addition. Subsequent to glutamate exposure, a progressive decrease of respiratory control ratio to almost 1 was found within the following 3-5 h. The addition of extra calcium had no effect per se on oxygen consumption by cell homogenate.