FMLP causes eicosanoid-dependent vasoconstriction and edema in lungs from endotoxin-primed rats.
FMLP causes eicosanoid-dependent vasoconstriction and edema in lungs from endotoxin-primed rats.
复制标题
FMLP 会导致内毒素引发的大鼠出现类二十烷酸依赖性血管收缩和肺部水肿。
DOI：
10.1164/ajrccm/145.3.701
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Murphy,RC
中科院分区：
文献类型：
--
作者：
Voelkel,NF;Czartolomna,J;Simpson,J;Murphy,RC
Recruitment of inflammatory cells to the lung capillaries has been proposed as an important step in the sequence of events that lead to acute lung injury. Frequently, In the clinical setting, bacteremia and sepsis syndrome precede the acute lung failure and endotoxin priming may represent a comparable paradigm, useful for exparimental pursuit. Following addition of the chemotactic tripeptide FMLP (10-'to 10-'M) to the cell· free, salt solution perfusate of Isolated rat lungs, only a small degree of vasoconstriction was observed. However, In lungs isolated from rats that received2 mg/kg Intraperitoneal Salmonella enterlfldls endotoxin 2 h before lung perfusion, FMLP dose dependently caused a large, transient pUlmonary pressor response, edema formation, and release of large amounts of thromboxane and leukotrlene B•• Since In" ifro priming with endotoxin, direct vascular Injury by neutrophil elastase, nor direct stimulation with FMLP of pulmonary artery rings from endotoxin-pretreated rats, mimicked the effects of In,, 1" 0 endotoxin priming, we conclude that the presence of Inflammatory cells In the lung capillaries accounted for the large amount of eicosanolds produced by the lungs after FMLPstimulation. In fact, by retrograde lavageof the lung circulation with a collagenase solution, previously adherent cell clumps were mobilized and identified. These cell Clumps, composed of red blood cells, neutrophlls, and platelets, were not seen
