High global diversity of cycloviruses amongst dragonflies
High global diversity of cycloviruses amongst dragonflies
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DOI：
10.1099/vir.0.052654-0
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发表时间：
2013-08-01
影响因子：
3.8
通讯作者：
Varsani, Arvind
中科院分区：
文献类型：
--
作者：
Dayaram, Anisha;Potter, Kristen A.;Varsani, Arvind
圆环病毒科的成员，特别是圆环病毒属，被认为只感染脊椎动物;然而，最近在昆虫中发现了同一科下的姐妹组成员，即拟议的圆环病毒属。为了探索环状病毒的多样性，更好地了解这些新的ssDNA病毒的进化，在这里，我们提出了五个环状病毒分离的三个种（Orthetrum sabina，Xanthocnemis zealandica和Rhionaeschna napa）收集在澳大利亚，新西兰和美国，分别。这5种病毒的基因组与其他环状病毒的基因组结构相似，都是一个约1.7kb的环状基因组和两个主要的双向转录ORF。将本研究期间收集的基因组序列数据与公共数据库中可用的所有环状病毒基因组相结合，以鉴定基因间区域中的保守基序和调控元件，并确定其基因组中的多样性和重组区域。这里报道的基因组代表了四种不同的环状病毒物种，其中三种是新的。我们的研究结果证实，环状病毒在有翼昆虫种群中广泛传播;迄今为止，在蜻蜓中发现的8种不同的环状病毒中，其中一些表现出广泛的地理分布。分离分析揭示了环状病毒之间的种内和种间重组事件，包括从不同来源（例如山羊肉和人类粪便）回收的基因组。类似于其他特征明确的环状ssDNA病毒，重组可能在环状病毒进化中发挥重要作用。
Members of the family Circoviridae, specifically the genus Circovirus, were thought to infect only vertebrates; however, members of a sister group under the same family, the proposed genus Cyclovirus, have been detected recently in insects. In an effort to explore the diversity of cycloviruses and better understand the evolution of these novel ssDNA viruses, here we present five cycloviruses isolated from three dragonfly species (Orthetrum sabina, Xanthocnemis zealandica and Rhionaeschna multicolor) collected in Australia, New Zealand and the USA, respectively. The genomes of these five viruses share similar genome structure to other cycloviruses, with a circular similar to 1.7 kb genome and two major bidirectionally transcribed ORFs. The genomic sequence data gathered during this study were combined with all cyclovirus genomes available in public databases to identify conserved motifs and regulatory elements in the intergenic regions, as well as determine diversity and recombinant regions within their genomes. The genomes reported here represent four different cyclovirus species, three of which are novel. Our results confirm that cycloviruses circulate widely in winged-insect populations; in eight different cyclovirus species identified in dragonflies to date, some of these exhibit a broad geographical distribution. Recombination analysis revealed both intra- and inter-species recombination events amongst cycloviruses, including genomes recovered from disparate sources (e.g. goat meat and human faeces). Similar to other well-characterized circular ssDNA viruses, recombination may play an important role in cyclovirus evolution.