Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody.
Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody.
复制标题
兔淋巴细胞单克隆抗体：T 细胞特异性抗体的制备和表征。
DOI：
10.1016/0161-5890(81)90003-1
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发表时间：
1981
影响因子：
3.6
通讯作者：
Knight,KL
中科院分区：
文献类型：
--
作者：
McNicholas,JM;Raffeld,M;Loken,MR;Reiter,H;Knight,KL
用兔脾和肠系膜淋巴结（MLN）细胞免疫小鼠的脾细胞与突变小鼠骨髓瘤细胞融合。获得了26个能与兔淋巴样细胞发生反应的克隆。通过膜免疫荧光，如目视分析和荧光激活细胞分选仪，这些克隆之一，9AE 10，产生的抗体与几乎所有的胸腺细胞（> 90%）和46 - 78%的脾，MLN和外周血淋巴细胞反应。用9AE 10单克隆抗体（MAb）和抗IG的双膜免疫荧光法显示，脾、MLN和外周血中的9AE 10+和IG+细胞是不同的、不重叠的群体。因此，9AE 10 MAb是T细胞特异性抗体，9AE 10 MAb也与大多数脑细胞和约30%的骨髓细胞反应。9AE 10单抗识别的抗原的生化分析表明，该抗原是一种表观分子量为25，000的糖蛋白。这些数据表明9AE 10 MAb可能针对Thy-1样抗原。
Spleen cells of mice immunized with rabbit spleen and mesenteric lymph node (MLN) cells were fused with mutant mouse myeloma cells. Twenty-six clones which react with rabbit lymphoid cells were obtained. By membrane immunofluorescence, as analysed visually and by the fluorescence-activated cell sorter, one of these clones, 9AE10, produced an antibody that reacted with nearly all thymocytes (> 90%) and with from 46 to 78% of spleen, MLN and peripheral blood lymphocytes. Double-membrane immunofluorescence with the 9AE10 monoclonal antibody (MAb) and anti-Ig showed that 9AE10+and Ig+cells of spleen, MLN and peripheral blood were distinct and non-overlapping populations. Thus, the 9AE10 MAb is a T-cell-specific antibody.The 9AE10 MAb also reacted with most brain cells and with approximately 30% of bone marrow cells. Biochemical analysis of the antigen recognized by the 9AE10 MAb indicated that the antigen is a glycoprotein with an apparent molecular weight of 25,000. These data indicate that the 9AE10 MAb may be directed against a Thy-1-like antigen.