Differential behavioral and neurochemical effects of exercise, reboxetine and citalopram with the forced swim test
Differential behavioral and neurochemical effects of exercise, reboxetine and citalopram with the forced swim test
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DOI：
10.1016/j.lfs.2009.02.005
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发表时间：
2009-04-24
期刊：
影响因子：
6.1
通讯作者：
Russo-Neustadt, Amelia
中科院分区：
文献类型：
--
作者：
Arunrut, Teda;Alejandre, Hilda;Russo-Neustadt, Amelia
目的：在这项研究中，我们研究了短期运动是否会促进海马脑源性神经营养因子（BDNF）的表达，也会增强Porsolt强迫游泳试验（FST）的活性，这是一种评估抗抑郁药物疗效的模型。我们还希望确定运动联合抗抑郁药是否比单独干预更有效地改变FST中的行为。与此同时，我们还预计这些干预措施将保持应激后BDNF水平，并且设计用于选择性增强去甲肾上腺素能或血清素能神经传递的抗抑郁药（分别为瑞波西汀或西酞普兰）将对行为和BDNF表达产生不同的影响。主要方法：雄性Sprague-Dawley大鼠分别给予运动（自主转轮跑）、瑞波西汀、西酞普兰或运动联合抗抑郁药治疗1周。在这段时间结束时，每个治疗组的一部分动物进行了FST。然后通过原位杂交定量海马BDNF mRNA的应激后水平。主要发现：我们的研究结果表明，虽然运动和抗抑郁治疗都保留了应激后海马BDNF mRNA的水平，但每种干预都会导致FST中独特的行为特征。我们发现抗抑郁药物治疗增加了FST的游泳时间，但运动减少了游泳时间。瑞波西汀加运动的组合会增加攀岩和潜水的次数，而西酞普兰加运动则会减少这些行为。意义：FST期间的积极行为虽然是抗抑郁药物所特有的，但可能并不反映海马BDNF表达的增加或其他与生存相关的益处。(C) 2009爱思唯尔公司版权所有。
Aims: In this study, we investigated whether short-term exercise, known to promote hippocampal brain-derived neurotrophic factor (BDNF) expression, would also enhance activity in the Porsolt forced swim test (FST), a model for assessing antidepressant efficacy. We also wished to determine whether exercise combined with antidepressants would be more effective at modifying behavior in the FST than either intervention alone. In parallel with this, we also expected that these interventions would preserve post-stress levels of BDNF, and that antidepressants designed to selectively enhance noradrenergic or serotonergic neurotransmission (reboxetine or citalopram, respectively) would have differential effects on behavior and BDNF expression.Main methods: Male Sprague-Dawley rats were treated with exercise (voluntary wheel running), reboxetine, citalopram, or the combination of exercise and each antidepressant, for I week. At the end of this period, a subset of animals from each treatment group underwent the FST. Post-stress levels of hippocampal BDNF mRNA were then quantified via in situ hybridization.Key findings: Our results indicate that while both exercise and antidepressant treatment preserved post-stress levels of hippocampal BDNF mRNA, each intervention led to a unique behavioral profile in the FST. We found that antidepressant treatment increased swimming time in the FST, but that exercise decreased swimming time. While the combination of reboxetine-plus-exercise led to an increase in climbing and diving, citalopram-plus-exercise reduced these behaviors.Significance: It is possible that active behaviors during the FST, though specific to antidepressant medications, may not reflect increased hippocampal BDNF expression or other survival-associated benefits. (C) 2009 Elsevier Inc. All rights reserved.