MEMBRANE AND CYTOPLASMIC PROTEINS ARE TRANSPORTED IN THE SAME ORGANELLE COMPLEX DURING NEMATODE SPERMATOGENESIS
MEMBRANE AND CYTOPLASMIC PROTEINS ARE TRANSPORTED IN THE SAME ORGANELLE COMPLEX DURING NEMATODE SPERMATOGENESIS
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DOI：
10.1083/jcb.102.5.1787
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发表时间：
1986-05-01
影响因子：
7.8
通讯作者：
WARD, S
中科院分区：
文献类型：
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作者：
ROBERTS, TM;PAVALKO, FM;WARD, S
秀丽隐杆线虫（Caenorhabditis elegans）的假足精子发育过程中，蛋白质合成在分化完成前停止。利用结合在精子表面的单克隆抗体SP56和识别主要精子细胞质蛋白（MSP）的TR20的胶体金偶联物，标记嵌入在Lowicryl K4M中的睾丸薄片，以跟踪多肽从精子发生早期的合成到它们分离到成熟细胞的特定区室。这两种抗原都在原代精母细胞中合成，并组装成一个独特的双细胞器，即纤维体-膜细胞器（FB-MO）复合物。然而，抗原定位在这个FB-MO复合物的不同区域。如前所述，蛋白质组装到FB-MO复合体中，使减数分裂后的精子中膜和细胞质成分都能集中。然后，这种瞬时结构的逐步分解确保了每种成分在成熟精子中的最终目的地：纤维体中的MSP细丝解聚，将MSP释放到细胞质中，膜细胞器与质膜融合，将SP56抗原传递到表面。
During the development of pseudopodial spermatozoa of the nematode, Caenorhabditis elegans, protein synthesis stops before differentiation is completed. Colloidal gold conjugates of monoclonal antibody SP56, which binds to the surface of spermatozoa, and TR20, which recognizes the major sperm cytoplasmic protein (MSP), were used to label thin sections of testes embedded in Lowicryl K4M in order to follow polypeptides from their synthesis early in spermatogenesis to their segregation to specific compartments of the mature cell. Both antigens are synthesized in primary spermatocytes and are assembled into a unique double organelle, the fibrous body-membranous organelle (FB-MO) complex. However, the antigens are localized in different regions of this FB-MO complex. As described in detail, the assembly of proteins into the FB-MO complex allows both membrane and cytoplasmic components to be concentrated in the spermatids after meiosis. Then, the stepwise disassembly of this transient structure ensures delivery of each component to its final destination in the mature spermatozoan: MSP filaments in the fibrous body depolymerize, releasing MSP into the cytoplasm and the membranous organelles fuse with the plasma membrane, delivering SP56 antigen to the surface.