Neutrophil extracellular traps are increased in cancer patients but does not associate with venous thrombosis
Neutrophil extracellular traps are increased in cancer patients but does not associate with venous thrombosis
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DOI：
10.21037/cdt.2017.08.01
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发表时间：
2017-12-01
影响因子：
2.4
通讯作者：
Albadawi, Hassan
中科院分区：
文献类型：
--
作者：
Oklu, Rahmi;Sheth, Rahul A.;Albadawi, Hassan
背景资料：一个单中心，前瞻性的组织为基础的研究进行了调查之间的关联中性粒细胞胞外陷阱（NETs）和静脉血栓栓塞性疾病的恶性肿瘤patients.Methods：血浆收集65例患者，其中27例癌症患者和38例年龄匹配的非癌症患者。定量血浆NET、循环游离DNA（cfDNA）、DNA酶-1、核酸内切酶-G、核酸内切酶活性和凝血酶-抗凝血酶III（达特）复合物水平。还比较了实验室检查值。此外，NETs检测和定量进行荧光免疫组织化学（IHC）在组织库肿瘤切片和新鲜的人静脉血栓来自癌症patients.Results：癌症患者的血浆样品中含有较高水平的核小体（P=0.0009）和cfDNA（P=0.0008）相比，非癌症组。蛋白质印迹分析显示，与非癌症患者相比，癌症患者血浆样本中DNase- 1蛋白水平显着降低（P=0.016），与较低的核酸酶活性（P=0.03）平行。来自癌症患者的血栓组织以及来自肝癌和肺癌的肿瘤组织也显示出显著水平的NET。然而，在癌症患者的NET水平的增加并没有与达特复合物激活或癌症patient.Conclusions静脉血栓形成的患病率：需要进一步的研究，以确定NET作为促凝血剂在人类血栓形成的作用。
Background: A single center, prospective tissue-based study was conducted to investigate an association between neutrophil extracellular traps (NETs) and venous thromboembolic disease in patients with malignancy.Methods: Plasma was collected from 65 patients in which 27 were cancer patients and 38 were agematched non-cancer patients. Plasma NETs, circulating free DNA (cfDNA), DNase- 1, endonuclease-G, endonuclease activity and thrombin-antithrombin III (TAT) complex levels was quantified. Laboratory values were also compared. Additionally, NETs detection and quantification was performed with fluorescent immunohistochemistry (IHC) in tissue-banked tumor sections and fresh human venous thrombus derived from cancer patients.Results: Plasma samples from cancer patients contained higher levels of nucleosomes (P=0.0009) and cfDNA (P=0.0008) compared to the non-cancer group. Western blot analysis revealed significantly lower DNase- 1 protein levels (P=0.016) that paralleled lower nuclease activity (P=0.03) in plasma samples from cancer patients compared to non-cancer patients. Thrombus tissue from cancer patients and tumor tissue from liver and lung cancer also showed marked levels of NETs. However, increased levels of NETs in cancer patients did not correlate with TAT complex activation or prevalence of venous thrombosis in cancer patients.Conclusions: Further studies are warranted to determine the role of NETs as a procoagulant in human thrombosis.