Purification of poly(A)-messenger ribonucleic acid by reversed-phase high-performance liquid chromatography.
Purification of poly(A)-messenger ribonucleic acid by reversed-phase high-performance liquid chromatography.
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通过反相高效液相色谱法纯化聚(A)-信使核糖核酸。
DOI:
10.1016/s0021-9673(01)90908-2
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发表时间:
1983
期刊:
影响因子:
--
通讯作者:
Capp,MW
中科院分区:
文献类型:
--
作者:
Simonian,MH;Capp,MW
Polyadenylated messenger ribonucleic acid [poly(A)-mRNA] was purified by reversed-phase high-performance liquid chromatography (RP-HPLC). Partially purified poly(A)-mRNA was prepared from rat liver polysomes by magnesium precipitation followed by affinity chromatography on an oligo(dT) cellulose column. Translatable poly(A)-mRNA, as assayed byin vitrotranslation in a rabbit reticulocyte system, was resolved from non-translatable RNA by RP-HPLC. The separation appears to be due to the presence of poly(A) residues on the 3′ terminus of the mRNA.
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影响因子:
2.9
作者:
R. Palmiter
通讯作者:
R. Palmiter
影响因子:
4.1
作者:
E. Nice;M. Capp;M. O'hare
通讯作者:
M. O'hare
DOI:
--
发表时间:
1982
期刊:
影响因子:
--
作者:
T. Narihara;Y. Fujita;T. Mizutani
通讯作者:
T. Mizutani
影响因子:
4.8
作者:
Simonian,MH;Gill,GN
通讯作者:
Gill,GN
影响因子:
64.5
作者:
B. Lewin
通讯作者:
B. Lewin