IDENTIFICATION OF A CELLULAR TRANSCRIPTION FACTOR INVOLVED IN E1A TRANSACTIVATION
IDENTIFICATION OF A CELLULAR TRANSCRIPTION FACTOR INVOLVED IN E1A TRANSACTIVATION
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DOI：
10.1016/0092-8674(86)90386-7
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发表时间：
1986-04-25
期刊：
影响因子：
64.5
通讯作者：
NEVINS, JR
中科院分区：
文献类型：
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作者：
KOVESDI, I;REICHEL, R;NEVINS, JR
我们已经使用凝胶试验来检测腺病毒感染细胞的核提取液中的转录因子，该转录因子与腺病毒E2启动子(E1a可诱导启动子)相互作用。在足迹分析中，因子与启动子的结合保护了-33和-74之间的序列不被DNA酶切割。这一区域也受到保护，不受核酸外切酶III的消化。这些序列与先前被证明对启动子活性和E1a刺激是必需的那些序列一致。该因子可以在未感染细胞的提取液中检测到，尽管水平大大降低。感染细胞中因子水平的增加需要一个功能正常的E1a基因。这些结果表明，E2结合活性是一种细胞转录因子，其浓度或结合活性随着E1a基因产物的作用而增加。
We have employed a gel assay to detect a transcription factor in nuclear extracts of adenovirus-infected cells that interacts with the adenovirus E2 promoter, an E1A inducible promoter. Binding of the factor to the promoter protected sequences between -33 and -74 from DNase cleavage in a footprint assay. This region was also protected from exonuclease III digestion. These sequences coincide with those previously shown to be essential for promoter activity and E1A stimulation. The factor could be detected in extracts of uninfected cells, although at greatly reduced levels. The increased level of factor in infected cells required a functional E1A gene. These results suggest that the E2 binding activity is a cellular transcriptional factor, the concentration or binding activity of which increases as a result of the action of the E1A gene product.