Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei.
Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei.
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DOI：
10.1111/j.1574-6968.2000.tb09432.x
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发表时间：
2000-12
影响因子：
2.1
通讯作者：
Jianfeng Wang;Guiling Li;Huaying Lu;Z. Zheng;Yaojian Huang;Wen-jin Su
中科院分区：
文献类型：
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作者：
Jianfeng Wang;Guiling Li;Huaying Lu;Z. Zheng;Yaojian Huang;Wen-jin Su
二萜类紫杉醇是一种重要的抗肿瘤药物，临床应用广泛。对从南方红豆杉（Taxus mairei）中分离到的一株产紫杉醇内生真菌（TF5）进行了鉴定，并对其抗癌活性进行了研究。根据真菌培养物的形态、产孢机制和孢子特征，将菌株TF 5鉴定为一种假单孢菌属（Acidularia sp.）。利用薄层色谱、高效液相色谱、紫外光谱和质谱等分析手段，研究了菌株TF5在马铃薯葡萄糖液体培养基中的生长情况。从TF 5培养物的有机提取物中分离得到的真菌紫杉醇在体外对KB和P388癌细胞具有较强的细胞毒活性。用免疫荧光和电子显微镜观察到，真菌紫杉醇增强了培养细胞中微管的稳定性和成束性，并在体外诱导微管蛋白聚合，与真正的紫杉醇相似。
The diterpenoid taxol is an important anticancer agent used widely in the clinic. The purpose of this work was to identify a taxol-producing endophytic fungus (strain TF5) isolated from Taxus mairei and study its anticancer activities. Strain TF5 was identified as a Tubercularia sp. according to the morphology of the fungal culture, the mechanism of spore production and the characteristics of the spores. Strain TF5 produced taxol, when grown in potato dextrose liquid medium and analyzed by thin layer chromatography, high performance liquid chromatography, ultraviolet and mass spectrometry. The fungal taxol, which was isolated from the organic extract of the TF5 culture, had strong cytotoxic activity towards KB and P388 cancer cells in vitro, tested by the MTT assay. Observed with immunofluorescence and electron microscopy, the fungal taxol enhanced microtubule stability and bundling in culture cells and induced tubulin polymerization in vitro similar to the authentic taxol.