Pharmacological inhibition of Rho-kinase signaling with Y-27632 blocks melanoma tumor growth
Pharmacological inhibition of Rho-kinase signaling with Y-27632 blocks melanoma tumor growth
复制标题
DOI：
10.3892/or_00000708
复制
发表时间：
2010-03-01
期刊：
影响因子：
4.2
通讯作者：
Bryan, Brad
中科院分区：
文献类型：
--
作者：
Routhier, Alissa;Astuccio, Michelle;Bryan, Brad
Primarily through in vitro studies, the Rho-family of small GTPases and their effector proteins have been implicated in mediating oncogenic properties of cancer cells. We sought to determine if pharmacological inhibition of the RhoA effector proteins known as Rho-kinases (ROCK) with the small molecule inhibitor Y-27632 could inhibit melanoma in vitro and in vivo. We demonstrate that Y-27632 treatment of a panel of melanoma cells alters cellular morphology leading to spindly cells with decreased lamellipodia and increased filopodia formation. Y-27632 treatment decreases invasion and alters cell survival Of Cultured melanoma cells. IP injection of Y-27632 ill tumor-bearing mice resulted in a reduction ill melanoma tumor Volume compared to control treated mice. These findings Suggest that ROCK inhibition call reduce melanoma tumorigenicity.
