A high-fat diet generates alterations in nuclear receptor expression:: Prevention by vitamin A and links with cyclooxygenase-2 and β-catenin
A high-fat diet generates alterations in nuclear receptor expression:: Prevention by vitamin A and links with cyclooxygenase-2 and β-catenin
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DOI：
10.1002/ijc.21108
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发表时间：
2005-10-10
影响因子：
6.4
通讯作者：
Cassand, P
中科院分区：
文献类型：
--
作者：
Delage, B;Bairras, C;Cassand, P
流行病学研究表明，从脂肪中摄入高能量，导致超重，增加癌症发展的风险，并促进结肠癌的发生。因此，重要的是要了解哪些参数会在早期受到高脂肪饮食的影响，以便制定和改进保护性营养策略。本研究观察了高能量/高脂肪摄入对雄性Wistar大鼠结肠粘膜的影响。对异常隐窝病灶（ACF）进行编号，并评估环氧合酶-2（考克斯-2）和β-连环蛋白水平的变化。过氧化物酶体增殖物激活受体和视黄酸激活受体（Peroxisome proliferator-and retinoic-activated receptor，PPAR，RAR，RXR）是调节基因表达的关键转录因子。设计了一项短期研究，以评估是否可以在食欲亢进饮食（HLD）诱导的体重增加阶段开始时检测到核受体mRNA表达的变化。HLD消耗诱导了PPAR γ（-33.1%）和RAR β（-53.1%）mRNA表达的早期下调，同时伴随着考克斯-2（+45.5%）和β-连环蛋白（+84.56%）水平的增加以及ACF数量的增加（191.56 +/- 88.60 vs. 21.14 +/- 11.64，p
Epidemiologic studies suggest that intake of high energy from fat, inducing overweight, increases the risk of cancer development and promotes colon carcinogenesis. It is therefore important to understand which parameters are affected early on by a high-fat diet in order to devise and improve protective nutritional strategies. We investigated the effect of high energy/fat intake on colon mucosa of male Wistar rats induced by a single 1,2-dimethylhydrazine (DMH) injection. Aberrant crypt foci (ACF) were numbered and modifications in cyclooxygenase-2 (COX-2) and beta-catenin levels assessed. Peroxisome proliferator- and retinoic acid-activated receptors (PPAR and RAR, RXR) are key transcription factors regulating gene expression in response to nutrient-activated signals. A short-term study was designed to evaluate whether alterations in mRNA expression of nuclear receptors can be detected at the beginning of the weight gain phase induced by an appetizing hyperlipidic diet (HLD). HLD consumption induced early down-regulation of PPAR gamma (-33.1%) and RAR beta (-53.1%) mRNA expression concomitant with an increase in levels of COX-2 (+45.5%) and beta-catenin (+84.56%) and in the number of ACF (191.56 +/- 88.60 vs. 21.14 +/- 11.64, p