Equine papillomavirus type 1: complete nucleotide sequence and characterization of recombinant virus-like particles composed of the EcPV-1 L1 major capsid protein
Equine papillomavirus type 1: complete nucleotide sequence and characterization of recombinant virus-like particles composed of the EcPV-1 L1 major capsid protein
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DOI：
10.1016/j.bbrc.2004.09.154
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发表时间：
2004-11-19
影响因子：
3.1
通讯作者：
Van Ranst, M
中科院分区：
文献类型：
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作者：
Ghim, SJ;Rector, A;Van Ranst, M
马乳头状瘤病毒I型（EcPV-1）是从马的皮肤乳头状瘤（最常见的肿瘤）中分离出来的。测定了EcPV-1全基因组序列和基因组结构。系统发育分析表明，EcPV-1是一种近根乳头状瘤病毒，与纤维乳头状瘤病毒和良性皮肤乳头状瘤病毒只有较远的关系。为了产生EcPV-1病毒样颗粒（VLP），使用重组杆状病毒载体在昆虫细胞中表达EcPV-1 L1主要衣壳蛋白。自组装的EcPV-1 VLP在形态上与野生型乳头瘤病毒病毒粒子无法区分。开发了针对完整和变性EcPV-1 VLP的单克隆抗体。当通过ELISA测试时，针对完整的（#18）和一些针对变性的EcPV-1 VLP（#16）产生的所有单克隆抗体仅与完整的EcPV 1 VLP反应，证明VLP在其表面上携带类型特异性构象以及线性表位。重组EcPV-1 VLP提供了预防和根除马皮肤乳头状瘤病的非感染性疫苗的潜力。(C)2004年爱思唯尔公司All rights reserved.
Equus caballus papillomavirus type I (EcPV-1) was isolated from a cutaneous papilloma, the most common neoplasm in horses. The complete EcPV-1 nucleotide sequence and genomic organization were determined. Phylogenetic analysis showed that EcPV-1 is a close-to-root papillomavirus, with only distant relationships to the fibropapillomaviruses and the benign cutaneous papillomaviruses. To produce EcPV-1 virus-like particles (VLPs), the EcPV-1 L1 major capsid protein was expressed in insect cells using a recombinant baculovirus vector. The self-assembled EcPV-1 VLPs were morphologically indistinguishable from wild type papillomavirus virions. Monoclonal antibodies were developed against intact and denatured EcPV-1 VLPs. When tested by ELISA, all monoclonal antibodies produced against intact (#18) and some against denatured EcPV-1 VLPs (#16) reacted with intact EcPV1 VLPs only, demonstrating that the VLPs carry type-specific conformational as well as linear epitopes on their surface. Recombinant EcPV-1 VLPs offer the potential of a noninfectious vaccine to prevent and eradicate equine cutaneous papillomatosis. (C) 2004 Elsevier Inc. All rights reserved.