Prevalence, pathogenicity, and serotypes of Vibrio parahaemolyticus in shrimp from Chinese retail markets
Prevalence, pathogenicity, and serotypes of Vibrio parahaemolyticus in shrimp from Chinese retail markets
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DOI：
10.1016/j.foodcont.2014.04.042
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发表时间：
2014-12-01
期刊：
影响因子：
6
通讯作者：
Wu, Kui
中科院分区：
文献类型：
--
作者：
Xu, Xiaoke;Wu, Qingping;Wu, Kui
副溶血性弧菌是亚洲国家最常见的食源性致病菌之一。以前曾报道过中国零售虾被副溶血性弧菌污染，这种污染的虾与食源性疾病的暴发有关。然而，到目前为止，中国零售虾中副溶血性弧菌的流行程度尚未确定。本研究旨在了解中国零售市场对虾中副溶血性弧菌的流行情况。2012年5月至2013年4月，在中国16个省（19个城市）的零售市场共获得虾类样本273份。最可能数法检出副溶血性弧菌103例（37.7%）。95.1%（98/103）的样本细菌密度小于100 MPN/g。从247株分离物中鉴定出5株trh阳性分离物，没有一株tdh阳性分离物。对247株病原菌进行多重pcr分型，除O9外，其余均检测到o -抗原血清型，其中以O-2型最常见（82株）。这是国内首次报道零售对虾中副溶血性弧菌的流行情况，本研究结果可用于国内对虾微生物风险评估。(C) 2014 Elsevier Ltd.版权所有。
Vibrio parahaemolyticus is one of the most common foodborne pathogens in Asian countries. V parahaemolyticus contamination of retail shrimp in China has been reported previously, and such contaminated shrimp have been linked to outbreaks of foodborne diseases. However, to date, the prevalence of V. parahaemolyticus in retail shrimp in China has not been determined. This study aimed at identifying the prevalence of V. parahaemolyticus in shrimps in Chinese retail market. From May 2012 to April 2013, a total of 273 shrimp samples was obtained from retail markets in 16 provinces (19 cities) of China. V. parahaemolyticus was detected in 103 (37.7%) of 273 samples by the most probable number method. Bacterial densities were less than 100 MPN/g in 95.1% (98/103) of the samples. Five trh-positive isolates were identified from 247 isolates, and none of the isolates were tdh-positive. In multiplex PCR-based O-antigen serotyping of these 247 pathogenic isolates, all O-antigen serotypes, except O9, were detected, and serotype O-2 was found to be the most prevalent (detected in 82 isolates). This is the first report on V. parahaemolyticus prevalence in retail shrimp in China, and the findings of this study can be used for microbiological risk assessment of shrimp in China. (C) 2014 Elsevier Ltd. All rights reserved.